Objective To study the microslructure of the nHA/PA66 membrane and investigate its cytocompatibility. Methods nHA/PA66 membrane was experimental group and expanded polytetrafluoroethylene (e-PTFE) membrane was control group. Light microscope and scanning electronic microscope(SEM) were used to observe the microstruelure of e-PTFE membrane and nHA/PA66 membrane. MG63 osteoblast-like cells were cultured on the two kinds of membrane and blank group. The cell proliferation was checked by MTT method and alkaline phosphatase(ALP) activity was detected by ELISA. SEM was used to observe the adhesion and proliferation of the MC63 osteoblast-like cells on the two kinds of membrane. Results The nHA/PA66 membrane was composed of the obverse face and the opposite face. The obverse face was porous and the opposite face was smooth. The e-PTFE membrane showed many tiny lined cracks ( l-5um in width and 5-20um in lengthen elliptic structure. The MTT assay and ALP measurement showed that there was no significant difference between each of the two membrane groups and the blank. The adhesion and proliferation of osteoblast-like cells on the nHA/PA66 membrane were better than the e-PTFE membrane. Conclusion nHA/PA66 membrane has no negative effects on the growth of osteoblast -like cells. nHA/PA66 membrane is bioeompatibility and its microstructure is appropriate for guided bone regeneration materials.%目的 观察纳米羟基磷灰石/聚酰氨66(nHA/PA66)复合膜的结构及其对成骨样细胞生物相容性的影响.方法 以膨体聚四氟乙烯膜(e-PTFE)为对照组,扫描电镜和光学显微镜下观察两组膜的结构.在两纽膜材料上分别接种MG63成骨样细胞并设空白对照.采用MTT法检测细胞的增殖活力,酶联免疫法检测细胞内碱性磷酸酶(ALP)表达,扫描电镜观察细胞在生物膜表面的增殖与粘附情况.结果 nHA/PA66膜分为正、反两面,正面为疏松多孔结构,反面为致密光滑、有少量微孔散在分布的结构.e-PTFE膜由成行排列,直径比较均一的长椭圆形的裂隙组成,正反面、断面结构基本一致.与空白对照组相比,MTT检测显示,nHA/PA66膜和e-PTFE膜对成骨样细胞增殖活力无显著性影响(P＞0.05).ALP活性也无显著性差异(P＞005).扫描电镜观察细胞在两种膜上生长良好,在nHA/PA66膜上细胞伸展更充分.结论 nHA/PA66膜对成骨样细胞生长无抑制作用,适于做引导骨再生膜材料,相比较ePTFE,其具有更优良的结构及生物学性能.
展开▼
