DNA-PKcs表达与乳腺癌临床病理特征关系的研究

摘要

Objective: To investigate the expression of DNA - PKcs and its relationship with clinicopathological characteristics in invasive breast cancer. Methods : DNA - PKcs and ER, PR, c - erhB - 2 protein expression were assessed on a tissue microarray containing 101 invasive breast cancer cases. Furthermore, age, tumor size, TNM staging, differentiation , lymph node metastasis and so on were analyzed. Results :The expression intensity of DNA PKcs protein in invasive breast cancer was significantly associated with tumor size( rs = - 0. 319 ,P = 0. 001 ) ,lymph node metastasis ( rs = - 0. 378 ,P = 0. 000 ) , and TNM stage( rs = - 0. 428 ,P = 0. 000 ). Furthermore , DNA - PKcs expression has a positive relationship with the expression of ER( rs = 0. 279 ,P =0. 005 ). However, there was no significant relationship between DNA - PKcs expression and age, differentiation and expression of PR, c - erbB - 2 in tumor cells. Conclusions : Low or negative expression of DNA - PKcs show poor prognosis and lymph node metastasis potential and it can be a prognostic marker of clinical outcome in breast cancer.%目的:研究DNA依赖蛋白激酶催化亚单位(DNA-PKcs)表达与浸润性乳腺癌临床病理特征的关系.方法:应用组织芯片技术和免疫组织化学方法检测101例浸润性乳腺癌中DNA-PKcs以及ER、PR、c-erbB-2的表达情况,采用χ2检验及Spearman秩和相关检验分析结果.结果:DNA-PKcs表达与浸润性乳腺癌的肿 瘤大小、淋巴结转移数量、TNM 分期均呈负相关(分别为rs=-0.319,P=0.001;rs=-0.378,P=0.000;rs =-0.428,P=0.000);与ER表达呈正相关(rs=0.279,P=0.005);与患者年龄、肿瘤组织学分级、及PR、c-erbB-2表达的关系无统计学意义(P＞0.05).结论:DNA-PKcs低表达与浸润性乳腺癌的进展及淋巴结转移关系密切,有可能成为预测乳腺癌预后重要的生物学指标.