Objective : To detect the expression of CD44v6 and MMP -2 in infiltrating ductal carcinoma, and explore their relationship with lymph node metastasis. Methods : The expression of CD44v6 and MMP -2 was studied in 100 cases of infiltrating ductal carcinoma by SP immunohistochemistry. Results: The positive expression of CD44v6 protein in normal breast tissue and infiltrating ductal carcinoma was 12. 5 ％ ( 5/40) , 87％ ( 87/100) respectively, and the positive rate of CD44v6 in patienta with lymph node metastasis(56/60) significantly higher than those without lymph node metastasis( P ＜ 0. 05 ) . The positive expression of MMP - 2 protein in normal breast tissue and infiltrating ductal carcinoma was 0( 0/40) ,94％ ( 94/100) respectively, and the positive rate of MMP - 2 in patients with lymph node metastasis ( 59/60) significantly higher than those without lymph node metastasis( P ＜ 0. 05 ) . Conclusion : CD44v6 and MMP - 2 protein are highly expressed in infiltrating ductal carcinoma and are all related with lymph node metastasis ( P ＜ 0. 05) , combined detection of CD44v6 and MMP - 2 is useful to estimation of infiltrating ductal carcinoma malignancy and metastatic potential.%目的:检测CD44v6、MMP-2在乳腺浸润性导管癌中的表达情况,探讨它们与淋巴结转移的关系.方法: 采用免疫组化SP法检测100例乳腺浸润性导管癌中CD44v6、MMP-2的表达情况.结果: CD44v6蛋白在正常乳腺组织及乳腺浸润性导管癌中的阳性表达率分别为12.5%(5/40)、87%(87/100),CD44v6在淋巴结转移组中的阳性表达率(56/60)明显高于无淋巴结转移组(P<0.05).MMP-2蛋白在正常乳腺组织及乳腺浸润性导管癌中的阳性表达率分别为0(0/40)、94%(94/100),MMP-2在淋巴结转移组中的阳性表达率(59/60)明显高于无淋巴结转移组(P<0.05).结论: CD44v6、MMP-2蛋白在乳腺浸润性导管癌组织高表达,且均与淋巴结转移有关(P<0.05),联合检测CD44v6与MMP-2有助于综合判断乳腺浸润性导管癌的恶性程度和转移潜能.
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