目的:探讨5-氮杂-2-脱氧胞苷(5-Aza-CdR)干预对胰腺癌细胞株PANC-1增殖和凋亡的影目的:探讨5-氮杂-2-脱氧胞苷(5-Aza-CdR)干预对胰腺癌细胞株PANC-1增殖和凋亡的影响及可能的机制.方法:用不同浓度的5-Aza-CdR处理PANC-1细胞株,未予干预的细胞为对照,采用台盼蓝染色、细胞计数法检测5-Aza-CdR干预前后细胞增殖能力的变化,FCM法检测干预前后细胞凋亡率的变化,caspase-3活性测定检测5-Aza-CdR诱导凋亡的能力.结果:5-Aza-CdR干预后,PANC-1细胞增殖明显受到抑制,细胞凋亡率明显提高,细胞内caspase-3活性明显增强(P＜0.05).结论:5-Aza-CdR可以明显抑制胰腺癌细胞株PANC-1的生长增殖,并诱导细胞发生凋亡,线粒体凋亡途径可能是其诱导凋亡的机制之一.%Objective: To investigate the effects of 5 - Aza - 2 - deoxycyt ( 5 - Aza - CdR ) on the proliferation and apoptosis of pancreatic carcinoma cell line Pane - 1, and explore its possible mechanisms. Methods: PANC - 1 cell line was treated by 5 - aza - CdR of different concentrations. Trapan blue exclusion cell count assay, flow cytometry and activity test of caspase -3 protein were used to determine the cell growth and apoptosis. Results; After treated by 5 - Aza - 2 - deoxycyt , the cell proliferation of PANC - 1 cell line was inhibited obviously, the apoptosis rate was in-creased significantly ( P <0. 05) , compared with the control group. The activity of caspase - 3 protein in cells was markedly enhanced after being treated by 5 - Aza - 2 - deoxycyt. Conclusion: 5 - Aza - 2 - deoxycyt can inhibit cell proliferation of PANC - 1 cell line,and induce apoptosis of PANC - 1 cell line, the mitochondrial pathway might be one of mechanisms inducing the apoptosis of PANC - 1 cells.
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