目的 研究细胞外信号调节激酶2(extracellular regulated kinase 2,ERK2)及其磷酸化状态(p-ERK1/2)在宫颈癌的表达情况,探讨二者与宫颈癌侵袭、转移的关系.方法 采用免疫组化(SP)法,检测80例宫颈癌组织、50例CIN组织及30例正常宫颈组织中ERK2和p-ERK1/2的表达.结果 ERK2和p-ERK1/2在宫颈癌中阳性表达率为75%和52.5 %,明显高于CIN组的34%和28%,以及正常宫颈组的13.33%和20%(P<0.01); ERK2和p-ERK1/2阳性表达率随临床分期的增高而增加(P<0.05),病理分级的降低而增加(P<0.01),有淋巴结转移者ERK2和p-ERK1/2阳性表达率明显高于无转移者(P<0.01);ERK2及p-ERK1/2在宫颈癌中的表达呈正相关(r=0.61,P<0.01).结论 ERK2及p-ERK1/2的表达与宫颈癌的生长、浸润、转移等生物学行为关系密切,在宫颈癌的发生发展过程中发挥重要作用.%Objective: To investigate the expression of ERK2 and p - ERK1/2 in cervical carcinoma and the relationship between the two factors and cervical cancer. Methods: The expression of ERK2 and p - ERK in human cervical cancer tissue of 80 cases, cervical intraepithelial neoplasia( CIN I , CIN II , CINIH )of 50 cases and normal cervical epithelium of 30 cases were detected by immunohistochemical SP technique. Results: The positive expressions of ERK2 and p - ERK1/2 were 80% and 52. 5% in cervical cancer respectively. There were significantly higher positive expression rates of ERK2 and p - ERK1/2 than those in the normal cervical epithelium and CIN( P <0. 01 ). The expression of p - ERK1/2 after therapy was lower than before the clinical therapy of the cervix( P < 0. 01 ), with the clinical stage and pathological grade increasing, the positive expression rates of ERK2 and P - ERK1/2 were significantly higher in cervical carcinomas, the positive expression rates of ERK2 and p - ERK1/2 in human cervical carcinomas without lymph node metastasis were lower than those with lymph node metastasis( P <0. 01 ). The expression of ERK2 positively related to p - ERK1/2 in cervical carcinomas( r = 0. 61, P < 0. 01 ). Conclusion: The expression levels of ERK2 and p - ERK1/2 were related significantly to the invasive growth and metastasis of human cervical carcinomas. They play important role during the occurrence and development of human cervical carcinomas.
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