男性乳腺癌血清VEGF水平与外周血循环肿瘤细胞的相关性及临床意义

摘要

目的:检测男性乳腺癌患者血清中VEGF及外周血CK19的表达水平,探讨二者的相互关系及临床意义.方法:应用ELISA法分别检测18例男性乳腺癌患者及8例健康男性志愿者血清中VEGF的水平,同时抽取相应的外周血标本,应用实时定量逆转录-聚合酶链反应(qRT-PCR)方法检测标本中CK19的表达水平,并进行相关性分析.结果:VEGF在男性乳腺癌患者血清中的表达水平明显高于正常健康男性,二者差异显著(P=0.015)；亚组分析表明,在腋淋巴结阳性的患者中,血清VEGF水平明显高于淋巴结阴性患者(P=0.026).CK19在健康男性外周血标本中未见表达,在男性乳腺癌患者外周血中,阳性表达5例(27.8％),而外周血中CK19阳性的男性乳腺癌患者血清VEGF水平显著高于CK19阴性的患者(130.34 ±77.18pg/ml vs58.01 ±52.43pg/ml,P=0.038).结论:男性乳腺癌患者血清VEGF水平与外周血微转移密切相关,提示VEGF可能参与了肿瘤细胞的侵袭和播散,可作为男性乳腺癌早期血行播散的血清学指标.%Objective;To explore the level of serum VEGF in male breast cancer and its clinical significance as well as the relationship with peripheral blood circulating tumor cells in the patients. Methods: Level of serum VEGF in 8 cases of healthy male volunteer and 18 cases of breast cancer were detected by ELISA. CK19 mRNA in peripheral blood was detected by qRT -PCR as an index of circulating tumor cells in the patients as well. Correlation between serum VEGF level and peripheral blood circulating tumor cells was analyzed. Results: Level of serum VEGF in male breast cancer were significantly higher compared with those in healthy volunteer (P=0.015).A subgroup analysis showed that in lymph node positive patients,level of serum VEGF were higher (P =0.026). CK19 mRNA was found in peripheral blood of 5 cases of male breast cancer and the positive rate was 27. 8% . However, it was not detected in that of healthy volunteers. Mean level of serum VEGF in CK19 positive and negative patients was 130. 34 ±77. 18 pg/ ml and 58.01 ±52. 43pg/ml, respectively. There was a significant difference between the two groups (P =0.038). Conclusion; Level of serum VEGF in male breast cancer was closely related with the peripheral blood circulating tumor cells. High level of serum VEGF may be a soluble biomarker for the early micrometastasis of male breast cancer.