乳腺癌患者外周血循环肿瘤细胞 MiR -21检测的临床意义

摘要

To study the expression of MiR - 21 in peripheral blood in patients with breast cancer,and explore its clinical significance for diagnosing and monitoring GC,and serve as a marker for the detection of CTCs in breast cancer patients. Methods:Peripheral blood samples were collected from 40 preoperative patients with breast cancer and 15 healthy volunteers. Steploop real - time reverse transcription - polymerase chain reaction(qRT - PCR) was used to detect the level of MiR - 21. Results:Patients with breast cancer displayed a significantly higher level of MiR - 21 in peripheral blood than those from controls(P ﹤ 0. 01). The MiR - 21 level was associated with the lymph node metastasis,TNM stage(P ﹤ 0. 05),was not associated with menstrual station,size of tumor,the expression of ER,PR and HER - 2(P ﹥ 0. 05). Conclusion:This study highlights the potential of the detection of MiR - 21 in pe-ripheral blood as a novel tool for diagnosis of GC and monitoring CTCs in breast cancer patients.%目的：研究乳腺癌患者血浆中的 MiR -21表达水平，初步探讨其在乳腺癌中作为循环肿瘤细胞（cir-culating tumor cells，CTCs）的临床意义。方法：采用基于 SYBR Green I 染料荧光分析的茎环实时定量逆转录聚合酶链式反应（qRT - PCR）分别检测40例乳腺癌患者及15例正常对照者外周血中 MiR -21的表达水平，了解 MiR -21表达与临床病理指标的关系。结果：乳腺癌患者血浆中的 MiR -21表达水平明显高于正常对照者（P ﹤0.01）。MiR -21表达与患者月经状况、肿瘤大小、雌激素受体（ER）、孕激素受体（PR）和人类表皮生长因子受体2（HER -2）表达均无明显关系（P ﹥0.05），与 TNM 分期、淋巴结转移相关（P ﹤0.05）。结论：茎环 qRT - PCR 是一种快速、准确、定量检测 MiR -21的方法，MiR -21是一个能有效辅助乳腺癌诊断和监测 CTCs 的潜在标志物。