KIT FOR DETECTING PERIPHERAL BLOOD CIRCULATING TUMOR CELL PD-L1 GENE MUTATION OF PATIENT WITH KIDNEY CANCER, AND DETECTION METHOD

摘要

Provided in the present invention are a kit for detecting a peripheral blood circulating tumor cell PD-L1 gene mutation of a patient with kidney cancer, and a detection method, which fall within the technical field of molecular biology. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a PD-L1 (human) primary antibody, goat anti-human IgG/HRP antibody, 0.1% Triton X-100, 0.3% H2O2 and a 6×PBS buffer solution. By means of the detection method provided by the present invention, the PD-L1 expression of a patient with advanced or recurrent kidney cancer can be detected without acquiring a tissue specimen by means of needle biopsy. The technology is a minimally invasive technology, can realize real-time detection, can avoid false positive results caused by edge effects possibly generated in the staining process, has good stability, reduces cell loss and improves the accuracy of detection.