H3K9ac、H3K4me2在卵巢浆液性上皮性肿瘤中的表达及意义

摘要

目的：探讨组蛋白 H3第9位赖氨酸残基乙酰化（H3K9ac）及组蛋白 H3第4位赖氨酸残基二甲基化（H3K4me2）在卵巢浆液性囊腺瘤、卵巢交界性浆液性囊腺瘤和卵巢浆液性囊腺癌组织中蛋白表达与浆液性上皮性卵巢癌发生、发展的关系及两者之间有无相关性。方法：应用免疫组织化学链霉菌抗生物素蛋白-过氧化酶连接法（SP 法）检测 H3K9ac、H3K4me2在30例卵巢浆液性囊腺瘤组织、30例卵巢交界性浆液性囊腺瘤组织及40例卵巢浆液性囊腺癌组织中的表达，观察表达差异并进行统计学处理。结果：H3K9ac 在卵巢浆液性囊腺瘤组织中阳性表达率为93.33%，在卵巢交界性浆液性囊腺瘤组织中阳性表达率为66.67%，在卵巢浆液性囊腺癌中阳性表达率为42.50%，良性、交界性、恶性组织间两两比较差异有统计学意义（ P ﹤0.05）。在不同手术病理分期、不同组织学分级的卵巢浆液性囊腺癌组织中 H3K9ac 的阳性表达率有统计学差异（P ﹤0.05）。H3K4me2在卵巢浆液性囊腺瘤组织中阳性表达率为90%，在交界性浆液性囊腺瘤组织中阳性表达率为73.33%，在卵巢浆液性囊腺癌中阳性表达率为52.50%，良性与交界性、交界性与恶性组织间比较差异无统计学意义（P ﹥0.05），良性与恶性组织间比较差异有统计学意义（ P ﹤0.05）。在不同手术病理分期、不同组织学分级的卵巢浆液性囊腺癌组织中 H3K4me2的阳性表达率无统计学差异（ P ﹥0.05）。H3K9ac、H3K4me2在卵巢浆液性囊腺癌组织中的表达呈正相关（r =0.732，P ﹤0.001）。结论：H3K9ac、H3K4me2的低表达与浆液性上皮性卵巢癌的发生、发展有关，有可能成为浆液性上皮性卵巢癌诊断和治疗的新靶点。%To explore the protein expression of H3K9ac and H3K4me2 in ovarian serous cystadenoma tissues,borderline serous cystadenoma tissues and serous cystadenocarcinoma tissues,their roles in occurrence and de-velopment of ovarian serous tumors,and the relationship between H3K9ac and H3K4me2. Methods:The expressions of H3K9ac,H3K4me2 in 30 cases of ovarian serous cystadenoma tissues,30 cases of ovarian borderline serous cystad-enoma tissues,40 cases of ovarian serous cystadenocarcinoma tissues were detected by immunology and histology chemistry SP. Results:There were significant differences in the expression of H3K9ac in ovarian serous cystadenoma tissues,ovarian borderline serous cystadenoma tissues,and ovarian serous cystadenocarcinoma tissues,and had correl-ativity with surgical pathologic stage and histological grade(P ﹤ 0. 05). The expression of H3K4me2 was significantly lower in ovarian serous cystadenocarcinoma than that in ovarian serous cystadenoma(P ﹤ 0. 05),but had no correlativ-ity with surgical pathologic stage and histological grade(P ﹥ 0. 05). H3K9ac had positive correlation with H3K4me2 in ovarian serous cystadenocarcinoma(r = 0. 732,P ﹤ 0. 001). Conclusion:H3K9ac and H3K4me2 protein expression is decreased in ovarian serous cystadenocarcinoma. The expression of H3K9ac and H3K4me2 might be correlated to the carcinogenesis and development of ovarian epithelial cancer. They might become the new target for diagnosis and treatment of ovarian epithelial cancer.