肾上腺素受体β1在肿瘤恶病质脂肪消耗中的作用

摘要

Objective:To investigate the implications of β1 -adrenergic receptor (ADRB1)in fat loss in cancer cachexia.Methods:ADRB1 gene was overexpressed in 3T3 -L1 cells and then differentiated by MDI -based crite-ria.The protein levels of ADRB1 and hormone sensitive lipase (HSL)were detected by Western blot.Results:3T3 -L1 differentiated completely in the control and mock groups,but failed in ADRB1 overexpression group,which could be attenuated by ADRB1 inhibitor.There was an increased protein level of HSL in ADRB1 overexpression group, which could be attenuated by ADRB1 inhibitor.Conclusion:ADRB1 leads to fat loss in cancer cachexia by stimula-ting HSL.%目的：研究 ADRB1在肿瘤恶病质脂肪消耗中的作用。方法：设立正常对照组、mock 组、ADRB1过表达组、抑制剂干预组。将 ADRB1基因稳定转染进3T3－L1前脂肪细胞，以 MDI 方案诱导分化，利用 Western blot 检测 ADRB1和 HSL 蛋白水平。结果：油红 O 染色证实，正常对照组和 mock 组前脂肪细胞能够分化， ADRB1过表达组无法分化，且可被 ADRB1抑制剂逆转。ADRB1过表达组中激素敏感性酯酶（HSL）蛋白表达增强，且可被 ADRB1抑制剂逆转。结论：ADRB1通过刺激甘油三酯（TG）分解导致恶病质脂肪消耗。