FGFR3、Rb 及 E2F -1在膀胱肿瘤组织中的表达及临床意义

摘要

Objective:To detect the function and clinical biological significance of FGFR3、Rb and E2F - 1 pro-tein in bladder tumor. Methods:Using different concentrations of FGFR3,Rb and E2F - 1 protein,to detect their ex-pression in 122 cases bladder tumor by SP immunohistochemical method. Results:The positive rate of FGFR3 and E2F - 1 in transitional cell cancer was 81. 5%(75 / 92),70. 7%(65 / 92),which was significantly higher than 56. 7%(17 / 30)and 46. 7%(14 / 30)in bladder papillary epithe - lioma(P = 0. 008;P = 0. 017). The positive expression rates of Rb protein in transitional cell cancer and papilloma were 68. 5%(63 / 92),93. 3%(28 / 30),the difference was statistically significant( P = 0 . 007 ). FGFR3 positively correlated with E2 F - 1(r = 1. 000,P < 0. 05);Rb negatively correlated with FGFR3(r = - 1. 000,P < 0. 05)and E2F - 1(r = - 1. 000,P < 0. 05). Conclusion:The up - regulation of FGFR3 and E2F - 1 expression and down - regulation of Rb expression may be associated with de-velopment of bladder cancer and can be a biological marker in diagnosis and judging malignancy degree of bladder cancer.%目的：探讨 FGFR3、Rb 及 E2F -1蛋白在膀胱肿瘤组织中的表达及临床意义。方法：以不同浓度的FGFR3、Rb 及 E2F -1蛋白，应用免疫组织化学 SP 法检测122例膀胱肿瘤组织中三种蛋白的表达。结果：FG-FR3和 E2F -1蛋白在膀胱移行细胞癌与乳头状瘤组中表达的阳性率分别为81.5％（75/92）、56.7％（17/30）和70.7％（65/92）、46.7％（14/30），移行细胞癌组的表达明显高于乳头状瘤组，组间差异有统计学意义（ P =0.008；P =0.017）。在移行细胞癌和乳头状瘤中 Rb 蛋白的阳性表达率分别为68.5％（63/92）和93.3％（28/30），移行细胞癌组的表达低于乳头状瘤组，组间差异有统计学意义（P =0.007）。Spearman 等级相关分析显示 FGFR3与 E2F -1蛋白在膀胱肿瘤组织中的表达呈正相关关系（r =1.000，P ＜0.05）。Rb 与 FGFR3、E2F