骨髓间充质干细胞表面分子 CD166和 CD271在胃组织中的表达

摘要

目的：探讨骨髓间充质干细胞表面分子 CD166和 CD271在正常胃黏膜、腺瘤及胃腺癌中的表达，初步探讨二者是否可以作为胃癌干细胞的标志物。方法：采用免疫组化染色 SP 法，对60例胃腺癌、10例正常胃组织标本和20例腺瘤的胃黏膜组织进行了骨髓间充质干细胞表面分子 CD166和 CD271的检测。结果：CD166在正常胃黏膜、不典型增生和胃癌中的表达逐渐降低，CD166在胃腺癌中的表达强度与胃癌的分化程度呈正相关（r =0.597，P ＜0.01），与浸润深度有关（P ＜0.01）。CD271在正常胃黏膜、不典型增生中几乎不表达，在胃癌中呈低表达，CD271在胃腺癌中的表达强度与浸润深度（P ＜0.01）、淋巴结转移有关（P ＜0.01），与患者的年龄、性别、肿瘤分化程度无关。结论：正常胃黏膜、腺瘤和腺癌的组织中存在着骨髓间充质干细胞表面分子 CD166的表达，阳性细胞的位置分布没有规律性，不是胃癌肿瘤干细胞的特异性标记物，CD271在正常胃黏膜、腺瘤中几乎不表达，在胃癌中呈低表达，阳性细胞的位置分布没有规律性，不能作为胃癌肿瘤干细胞的标记物。%Objective:To study the expression of bone marrow - derived mesenchymal stem cells maker CD166 and CD271 in nomal gastric mucosa,adenoma and adenocarcinoma. Methods:The expression of CD166 and CD271 in 10 cases of normal mucosa,20 cases of adenoma and 60 cases of gastric adenocarcinoma were detected by SP(strep-tavidin HRP)immunohistochemical staining. Results:The difference of CD166 in different gastric mucosa had signifi-cance. The expression of CD166 was positively correlated to differentiation and related with depth of infiltration(P <0. 01). The levels of expression of CD271 was significantly higher in adenocarcinoma than in nomal gastric mucosa and adenoma. The levels of expression of CD271 was related with lymph node metastasis(P < 0. 01)and depth of in-filtration(P < 0. 01). Conclusion:There were expression of some bone marrow - derived mesenchymal stem cells maker CD166 in gastric mucosa. The distribution of positive cells in gastric mucosa was irregular. CD166 and CD271 were not the specific stem cell maker of gastric adenocarcinoma.