条件复制腺病毒携带白细胞介素24增强 Hela 细胞放射敏感性

摘要

Objective:To evaluate the effects of the conditionally replicating adenovirus carrying interleukin - 24 combined with radiation on cervical cancer Hela cell. Methods:Hela cells were divided into four groups:PBS group, radiation group,ZD55 - IL - 24 group and ZD55 - IL - 24 with radiation group. The cell viability was assessed by MTT assay. The apoptotic rate of cells was assessed by Annexin V - FITC/ PI assay. The expression of Bax,Bcl - 2 was detected by Western blot. Results:PBS group,cell viability of radiation group,ZD55 - IL - 24 group and ZD55 -IL - 24 with radiation group were(7. 28 ± 1. 99)% ,(45. 16 ± 4. 12)% ,(33. 23 ± 2. 63 )% and(85. 27 ± 2. 03)% , which reveals cell viability of ZD55 - IL - 24 with radiation group was higher than other groups(P < 0. 05). Annexin V - FITC / PI revealed that the apoptotic rate of ZD55 - IL - 24 with radiation was higher than other groups(P< 0. 05). ROS level of ZD55 - IL - 24 with radiation was higher than other groups(P < 0. 05). The expression of Bax in ZD55 - IL - 24 with radiation group was higher than in other groups(P < 0. 05),while the expression of Bcl - 2 in ZD55 - IL - 24 with radiation group was lower than in other groups(P < 0. 05). Conclusion:The conditionally repli-cating adenovirus carrying interleukin - 24 can increase radiosensitivity of cervical cancer Hela cell.%目的：观察条件复制性腺病毒（ZD55）携带白介素24（ZD55- IL -24）单独与联合放射治疗对宫颈癌Hela 细胞的治疗效果。方法：体外培养宫颈癌 Hela 细胞，分成 PBS 组、放射治疗组、ZD55- IL -24组、ZD55-IL -24联合放射治疗组。采用 MTT 法检测细胞生长抑制率，Annexin V - FITC/ PI 双染法检测细胞凋亡率，流式细胞仪检测细胞活性氧 ROS 水平，Western blot 检测凋亡蛋白 Bax、Bcl -2表达。结果：MTT 法显示 PBS 组、放射治疗组、ZD55- IL -24组、ZD55- IL -24联合放射治疗组抑制率分别为（7.28±1.99）%、（45.16±4.12）%、（33.23±2.63）%、（85.27±2.03）%，ZD55- IL -24联合放射治疗组细胞抑制率大于其他三组（P