外用中药制剂对小鼠乳腺增生的治疗作用及对激素水平的影响

摘要

目的:研究外用中药制剂对小鼠乳腺增生的治疗作用并探讨其机制.方法:采用苯甲酸雌激素腹腔注射构建乳腺增生小鼠模型,分组为对照组、模型组与治疗组.对照组小鼠腹腔注射生理盐水,隔天1次,共30天;模型组与治疗组腹腔注射苯甲酸雌激素,隔天1次,共30天.造模完成后,治疗组小鼠乳房部涂抹外用中药制剂,每天1次共30天.测定各组小鼠体重变化,乳晕及乳头直径变化,血清雌激素(E2)、孕酮(P)指标,并取小鼠第二对乳房组织做病理切片观察.结果:治疗组与模型组小鼠比较,体重、乳晕及乳头直径均有统计学差异(P<0.05);治疗组小鼠病理切片HE染色显示乳腺增生组织结构明显改善,雌、孕激素水平较对照组无统计学差异(P>0.05),而与模型组对比则具有统计学差异(P<0.05).结论:外用中药制剂对小鼠乳腺增生具有改善作用,其机制可能是通过调节小鼠血清雌激素与孕酮水平来实现的.%Objective:To study and the effect of traditional Chinese medicine on the mammary gland hyperplasia in mice and to explore its mechanism.Methods:The model group was established by injecting estradiol benzoate into the abdominal cavity.The mice were divided into 3 groups(control,model and therapy).Normal saline was injected into the peritoneal cavity of the control group,every 2 days,altogether 15 times in 30 days.Estradiol benzoate was injected into the peritoneal cavity of the model group and therapy group,every 2 days,altogether 15 times in 30 days.After 30 days,the traditional Chinese medicine was smeared on the mammary of therapy group,once a day for 30 days.To observe the body weight changes of mice,areola and nipple diameter,serum estradiol(E2)and progesterone(P)index.And the pathological slices of the second couple breast tissue were observed.Results:We found the body weight and diameter of nipple of mice had differences between therapy group and model group(P<0.05).The level of estrogen and progesterone compared between the therapy group and control group showed no significant difference(P>0.05),and compared with the model group there had statistical difference(P<0.05).The treatment group mice pathological HE staining showed hyperplasia of the breast tissue structure was improved.Conclusion:The improvement effect of traditional Chinese medicine preparation for mammary gland hyperplasia in mice may be realized through regulating the mouse serum estrogen and progesterone level.