外阴鳞状细胞癌患者癌组织p16和DAPK基因组蛋白H3K27三甲基化状态水平研究

摘要

目的:研究外阴鳞状细胞癌患者癌组织p16和DAPK基因组蛋白H3K27三甲基化状态及mRNA表达水平.方法:采用染色质免疫共沉淀-实时定量聚合酶联反应(ChIP-qPCR)技术对8例外阴鳞状细胞癌患者癌组织和毗邻正常组织p16和DAPK基因组蛋白H3K27三甲基化状态进行定量分析;随后采用定量反转录聚合酶联反应(qRT-PCR)检测其基因的mRNA表达水平.结果:外阴鳞状细胞癌患者癌组织p16和DAPK基因组蛋白H3K27三甲基化水平增高;其三甲基化水平较毗邻正常组织有显著性差异(P<0.05);进一步表达分析显示p16和DAPK基因其mRNA表达降低.结论:外阴鳞状细胞癌患者癌组织p16和DAPK基因组蛋白H3K27三甲基化水平增高,组蛋白H3K27三甲基化异常参与外阴鳞状细胞癌的发生,极可能成为外阴鳞状细胞癌新的表观治疗靶点.%Objective:To investigate the histone H3 lysine 27 trimethylation(H3K27me3)of p16 and DAPK gene in vulvar squamous cell carcinoma(VSCC)tissue and mRNA expression. Methods:Chromatin immunoprecipitation-quantitative PCR was used to detect the histone H3 lysine 27 trimethylation status of p16 and DAPK gene in 8 cases of VSCC and the matched normal tissue. Expression analysis by qRT-PCR is performed on the p16 and DAPK gene. Results:Compared to that of normal tissue,p16 and DAPK gene in VSCC patients displayed increased H3K27me3. Increased H3K27me3 in p16 and DAPK gene lead to decreased mRNA expression. Conclusion:There are significant differences in H3K27me3 alterations between VSCC tissue and the matched normal tissue. H3K27me3 abnormalities in p16 and DAPK gene may be related with tumorigenesis of VSCC. Such novel findings may be become potential bio-marker or promising target for epigenetic-based VSCC therapies.