IMP3蛋白在骨肉瘤中的表达及其与肿瘤微血管密度的关系

摘要

目的:研究胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)在骨肉瘤中的表达及其与肿瘤微血管密度(MVD)的相关性,探讨二者与骨肉瘤发生发展的关系.方法:选取骨肉瘤患者62例,以骨软骨瘤患者术后石蜡切片20例为对照,采用免疫组化SP法分别检测IMP3和MVD在骨肉瘤和骨软骨瘤组织中的表达.结果:IMP3蛋白在骨肉瘤组织中的表达高于骨软骨瘤(P<0.05);IMP3蛋白在骨肉瘤的Enneking分期、肺转移、肿瘤复发和肿瘤直径中的表达差异具有统计学意义(均P<0.05);骨肉瘤微血管生成高于骨软骨瘤(P<0.05);CD34标记的MVD值在骨肉瘤的Enneking分期、肺转移、肿瘤复发和肿瘤直径中差异具有统计学意义(均P<0.05);IMP3蛋白表达和MVD值在骨肉瘤组织中呈正相关(r=0.259,P<0.05).结论:IMP3、MVD与骨肉瘤的发生及侵袭转移密切相关,可作为骨肉瘤恶性程度及预后的良好指标.%Objective:To study the the expressions of insulin - like growth factor ⅡmRNA binding protein 3 (IMP3)and tumor microvessel density (MVD)in osteosarcoma and to explore their relationship with the occurrence and development of osteosarcoma. Methods:The data of 62 cases of osteosarcoma were selected,and 20 cases of par-affin section in the patients with osteoenchondroma as control group. The expressions of IMP3 and MVD in osteosarco-ma and osteoenchondroma tissue were detected by immunohistochemistry SP method. Results:The expression of IMP3 protein in osteosarcoma was higher than that in osteoenchondroma (P < 0. 05). The expression of IMP3 protein was statistically significant in Enneking stage,lung metastasis,tumor recurrence and tumor diameter (P < 0. 05). The an-giogenesis of osteosarcoma was higher than that of the osteoenchondroma (P < 0. 05). MVD marked by CD34 was sta-tistically significant in Enneking stage,lung metastasis,tumor recurrence and tumor diameter (P < 0. 05). IMP3 ex-pression was positively correlated with MVD value in osteosarcoma tissues (r = 0. 259,P < 0. 05). Conclusion:IMP3 and MVD were closely related to the occurrence and metastasis of osteosarcoma,which can be used as good indicators of the malignant degree and prognosis of osteosarcoma.