ALPHAVIRUS-BASED EXPRESSION VECTORS EXPRESSING TUMOR SUPPRESSOR PROTEINS, METHOD FOR PREPARING THE SAME AND THE USE THEREOF TO SIMULTANEOUSLY EXPRESS VARIOUS TUMOR SUPPRESSOR PROTEINS, AND CONSEQUENTLY SUPPRESS TUMOR EFFECTIVELY

摘要

PURPOSE: Alphavirus-based expression vectors expressing tumor suppressor proteins, a method for preparing the same and the use thereof are provided, thereby simultaneously expressing various tumor suppressor proteins, and consequently suppressing tumor effectively. CONSTITUTION: The alphavirus-based expression vector expressing tumor suppressor proteins comprises a first tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a first subgenomic promoter; a second tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a second subgenomic promoter; and a third tumor suppressing gene or angiogenesis suppressing gene which is operably linked to a third subgenomic promoter, wherein the first, second and third tumor suppressing genes or angiogenesis suppressing genes are the same or different; the tumor suppressing gene is p53 gene or PTEN gene; the antigiogenesis suppressing gene is angiostatin gene; and the alphavirus-based expression vector expresses tumor suppressor proteins Semliki forest virus(SFV) and Sindbis virus.