槲皮素对人食管癌Eca109细胞增殖与凋亡的影响

摘要

Objective To observe the influence of quercetin on esophagus cancer Eca109 cell′s proliferation and apop-tosis. Methods Cultured esophagus cancer Eca109 cell in vitro. Used MTT method to test the influence of quercetin with differ-ent concentration,(40,80,160,320μmol/L) on esophagus cancer Eca109 cell′s proliferation after 24,48,72 h. Used flow cytome try to test the influence of quercetin with different concentration on esophagus cancer Eca109 cell′s apoptosis after 24 h. Results Quercetin had obvious inhibiting effect on esophagus cancer Eca109 cell′s proliferation,the more concentrated the quercetin was and the longer the time was,the greater the inhibiting effect was. The inhibiting rate of quercetin with different concentration on esophagus cancer Eca109 cell′s proliferation was clearly higher than control group,difference showing statistical significance(P<0.05). 24 h after applied uercetin with different concentration on esophagus cancer Eca109 cell,the apoptosis rate was tested to rise along with concentration increasement and was clearly higher than control group ,differences showing statistical significance (P<0.05). Conclusion Quercetin can distinctly inhibit esophagus cancer Eca109 cell′s proliferation,and induce esophagus cancer Eca109 cell′s apoptosis in a time and dosage dependent effect.%目的：观察槲皮素对人食管癌Eca109细胞增殖与凋亡的影响。方法体外培养人食管癌Eca109细胞，采用噻唑蓝（MTT）法检测用不同浓度的槲皮素（40、80、160、320μmol/L）作用24、48、72 h 对人食管癌 Eca109细胞增殖的影响；采用流式细胞仪检测不同浓度槲皮素作用24 h后对人食管癌Eca109细胞凋亡的影响。结果槲皮素对人食管癌Eca109细胞的增殖有明显抑制作用，随着槲皮素浓度的增加、作用时间的延长，抑制作用逐渐增强；不同浓度槲皮素作用Eca109细胞的增殖抑制率明显高于对照组，差异均有统计学意义（P<0.05）。不同浓度槲皮素对人食管癌Eca109细胞作用24 h后检测出的凋亡率随着浓度的增加而升高，且明显高于对照组，差异有统计学意义（P<0.05）。结论槲皮素能够明显地抑制人食管癌Eca109细胞的增殖，诱导人食管癌Eca109细胞的凋亡，并呈时间和剂量依赖效应。