牙龈缺损修复中钙结合蛋白和胶原酶-1的浓度变化

摘要

Objective To detect the levels of calbindin and collagenase-1 in the secretion at various time nodes of gingi-val defect after teeth extraction operation by the platelet-rich fibrin(PRF) membrane and collagen membrane, and to evaluate the ability of PRF on repairing the gingival defect and regulating the local inflammatory reaction. Methods Sixteen teeth needing to extract premolar teeth or molar and simultaneously conducting the site preservation in the Special Dental Implantation and Repair Clinical Center of the Chengdu Military Region from August 2013 to December 2014 were selected and divided into the PRF group and collagen membrane group,8 cases in each group. After extracting teeth,the tooth socket in the two groups was implanted by the bone substitute materials (Bio-Oss) and the surface was respectively covered by the PRF membrane and the collagen membrane. ELISA was used to detect the collagenase-1 and calbindin levels in the wound secretion on postoperative 3,7,14,21,28 d. The a-bility of PRF on the repair of gingival detection and regulation of local inflammatory reaction was dynamically evaluated. Results The calbindin level on postoperative 3,7 d in the collagen membrane group was higher than that in the PRF membrane group, and the difference was statistically significant (P<0.05),the calbindin level on postoperative 14,21,28 d in the collagen mem-brane group was slightly higher than that in the PRF membrane group ,but the difference was not statistically significant (P>0.05). The collagenase-1 level on postoperative 3,7 d in the PRF membrane group was significantly higher than that in the colla-gen membrane group,there are statistically significant difference(P<0.05),which on 14,21,28 d in the PRF membrane group was slightly higher than that in the collagen membrane group,the difference was not statistically significant(P>0.05). Conclusion PRF membrane can promote the repair of gingival defect and decrease local inflammatory reaction. PRF membrane is a biological activity regeneration platform material with some anti-infection ability and has a certain clinical value.%目的：检测富血小板纤维蛋白（PRF）膜与胶原膜修复拔牙术后牙龈缺损各时间节点的分泌物中钙结合蛋白和胶原酶-1的水平，评价PRF对牙龈缺损的修复和调节局部炎性反应的能力。方法选取2013年8月至2014年12月到成都军区机关医院种植修复临床专科中心就诊，需拔除磨牙并同期进行位点保存的患牙16例，随机分为PRF膜组和胶原膜组，各8例。拔牙后，牙槽窝内植入Bio-Oss骨替代材料，表面分别覆盖PRF膜或胶原膜。通过ELISA检测术区在术后3、7、14、21、28 d创面分泌物中胶原酶-1和钙结合蛋白的水平，动态评价PRF对牙龈缺损的修复和调节局部炎性反应的能力。结果钙结合蛋白水平在术后3、7 d胶原膜组均明显高于PRF膜组，差异均有统计学意义（P＜0.05）；术后14、21、28 d胶原膜组稍高于PRF膜组，但差异无统计学意义（P＞0.05）。胶原酶-1水平在术后3、7 d PRF膜组均明显高于胶原膜组，差异均有统计学意义（P＜0.05）；术后14、21、28 d PRF组稍高于胶原膜组，差异均无统计学意义（P＞0.05）。结论PRF能促进牙龈缺损的修复，减轻术区局部炎性反应。PRF膜是一种有一定抗感染能力的生物活性再生平台材料，有一定的临床价值。