阿托伐他汀对小鼠肾毒血清性肾炎保护作用的研究

摘要

Objective To investigate the interventional treatment effect of statins in mouse nephrotoxic serum nephritis. Methods The mouse nephrotoxic serum nephritis model was established by nephrotoxic serum (NTS) injection. Then mice in the treatment group were intraperitoneally injected by 1,10,25 mg/kg atorvastatin at 24 h after the model construction. After NTS plus atorvastatin treatment for 7 d,all mice were killed,theblood and 24 h urine were collected for detecting serum urea nitrogen (BUN) and urinary protein level. Furthermore the renal histopathologic changes were detected. Results Compared with the model group,the body weight increase in the atorvastatin treatment group was not obvious,the urinary protein level was decreased (P<0.01),serum BUN level was reduced (P<0.01),and the degree of nephritis pathologica change was alleviated. The protective effect of atorvastatin showed the dose dependent manner ,the protective effect tin the 10 mg/kg treatment group was strongest. Conclusion Atorvastatin can effectively alleviate urinary protein in the experimental animal ,improves the glomerular filtration function,and has the protective effect for mouse immune nephritis.%目的探讨他汀类药物对小鼠肾毒血清性肾炎的干预治疗作用。方法建立小鼠肾毒血清性肾炎模型，治疗组小鼠于注射肾毒血清（NTS）造模成功24 h内腹腔注射剂量分别为1、10、25 mg/kg阿托伐他汀。联合注射NTS+阿托伐他汀治疗7 d后处死所有小鼠，取血并收集24 h尿液，测定血清尿素氮（BUN）水平，尿蛋白含量，检查肾脏病理组织学变化。结果阿托伐他汀治疗组与模型组比较，实验小鼠体质量增加不明显，尿蛋白含量降低（P<0.01），血清BUN含量减少（P<0.01），肾炎病理改变程度轻。阿托伐他汀的保护作用呈剂量依赖性，10 mg/kg治疗组的保护作用最强。结论阿托伐他汀可有效地减轻实验动物尿蛋白，改善肾小球滤过功能，对小鼠免疫性肾炎具有保护作用。