NRP-1和NRP-2在结直肠癌组织中的表达水平及临床意义

摘要

目的：探讨神经纤毛蛋白1（neuropilin-1，NRP-1）和 NRP-2在结直肠癌组织中的表达水平及临床意义。方法选取2014年12月～2015年9月期间湖北省恩施州中心医院65例结直肠癌患者和65例肠息肉患者作为研究对象，选择于同期健康检查的65例健康女性作为正常对照组。采用实时荧光定量技术（RT-PCR）检测入组对象血清 NRP-1和 NRP-2水平。结果经 RT-PCR检测，结直肠癌组 NRP-1水平（36.51±4.62μg／ml）高于肠息肉组（8.19±4.08μg／ml）和健康对照组（7.92±4.05μg／ml），差异有统计学意义（F＝50.32，P＝0.000），结直肠癌组NRP-2 mRNA水平（24.59±3.26μg／ml）高于肠息肉组（5.47±1.11μg／ml）和健康对照组（5.19±1.05μg／ml），差异有统计学意义（F＝49.36，P＝0.000）。肠息肉组和健康对照组 NRP-1和 NRP-2 mRNA水平比较，差异无统计学意义（t＝1.15，1.07，P＝0.762，0.789）。经 RT-PCR检测，随着患者病理分期的增加、分化程度的降低，结直肠癌组血清 NRP-1和 NRP-2 mRNA水平呈升高趋势，伴肝转移和淋巴结转移的结直肠癌患者，血清 NRP-1和 NRP-2 mRNA水平高于未出现肝转移和淋巴结转移的结直肠癌患者（P＜0.05）。结直肠癌患者血清 NRP-1和 NRP-2 mRNA水平呈正相关（r＝0.415，P＝0.015）。结论结直肠癌血清NRP-1，NRP-2水平高于肠息肉及健康人群。血清NRP-1和NRP-2水平与结直肠癌的临床病理及预后密切相关，NRP-1， NRP-2可能共同起作用。%Objective To study serum NRP-1 and NRP-2 expression in colorectal cancer patients and its relationship with clinical pathology.Methods Between December 2014 and September 2015,in the Central Hospital of Enshi Autonomous Prefecture 65 cases of colorectal cancer patients and 65 patients with Intestinal polyps were selected as the research object, and between December 2014 and September 2015 60 cases of healthy women for health examination in the Central Hospital of Enshi Autonomous Prefecture were selected as normal control group.The real-time fluorescence quantitative technology (RT-PCR)was used to test serum NRP-1 and NRP-2 level.Results By RT-PCR detection,NRP-1 level of colorectal cancer group(36.51±4.62μg/ml)was higher than that of colon polyps group (8.19±4.08μg/ml)and the healthy controls (7.92 ±4.05μg/ml),and the difference was statistically significant (F= 50.32,P=0.000).NRP-2 mRNA level of colorectal cancer group (24.59±3.26μg/ml)was higher than that of colon polyps group (5.47±1.11μg/ml)and the healthy con-trols (5.19±1.05μg/ml),and the difference was statistically significant (F=49.36,P=0.000).And between NRP-1 and NRP-2 mRNA comparison of intestinal polyp group and healthy controls,there was no statistically significant difference (t=1.15,1.07,P=0.762,0.789).By RT-PCR detection,with the increase of patients with pathological staging and differentia-tion degree reducing,serum NRP-1 and NRP-2 mRNA of colorectal cancer group had the rise trend.NRP-1 and NRP-2 mR-NA of colorectal cancer patients with liver metastasis and lymph node metastasis were higher than colorectal cancer patients without liver metastasis and lymph node metastasis (P<0.05).Serum NRP-1 and NRP-2 mRNA levels of colorectal cancer patients were positively correlated (r=0.415,P=0.415).Conclusion Serum NRP-1 and NRP-2 levels of breast cancer were higher than that of benign breast lesions and healthy people.Serum NRP-1 and NRP-2 levels were closely related to the clinical pathology and prognosis of breast cancer.Breast cancer radical operation could inhibit the expression of miR-574-5p and improve the patient’s disease condition.Serum NRP-1 and NRP-2 levels of colorectal cancer were higher than intestinal polyps and healthy people.Serum NRP-1 and NRP-2 levels were closely associated with the clinical pathology and prognosis of colorectal cancer,and NRP-1 and NRP-2 could play the role together.