一株产纤维素酶真菌的筛选、鉴定及酶学性质初步研究

摘要

经过初筛和复筛从土样中分离出1株高产纤维素酶真菌SNB9,经形态学和ITS序列分析,鉴定为黑曲霉(Aspergullus niger).生长条件的测定显示该菌生长范围偏酸.发酵后纤维素酶的最适作用pH在 4.0～5.0,最适作用温度在 45～55 ℃.滤纸酶活为 9.29 U/mL,C -1酶活为 23.69 U/mL,CMCase酶活为 38.23 U/mL,β-葡萄糖苷酶活为 65.52 U/mL.发酵液中除了纤维素酶,还发现有辅助酶,包括木聚糖酶、淀粉酶、果胶酶、蛋白酶.%A cellulase high-producing fungus strain SNB9 was isolated from soil samples after proto-screening and re-screening and characterized as Aspergillus niger after morphological and ITS sequencing analyses. Growth conditions tests indicated that it grew on meta-acidic range. And the most suitable reactive pH of the cellulase was 4.0～5.0, and temperature 45～55 ℃. The activities of FPA, C1, CMCase, and β-glycosidase were 9.29 U/mL, 23.69 U/mL, 38.23 U/mL, 65.52 U/mL respectively. Besides cellulase, it was found there were some other auxiliary enzymes in the fermented broth, including xylanase, amylase, pectinase, and protease.