一株高产低温纤维素酶南极细菌的筛选、鉴定及酶学性质初步研究

摘要

从82株南极细菌中筛选出1株高产低温纤维素酶菌株N J64,通过16S rDNA序列分析对其进行鉴定,并对其产酶条件及酶学性质进行了初步研究.结果表明,NJ64菌株为假交替单胞菌属(Pseudoalteromonas sp.)；最适产酶条件为:培养时间72 h、初始pH值7.5、培养温度10℃；所产纤维素酶在pH值9.0左右、40℃条件下的酶活性最高,属于低温酶.%A high-producing cold-adapted cellulase antarctic bacterium named NJ64 was isolated from 82 an-tractic bacteria and identified by 16S rDNA sequence analysis. Its cellulase-producing conditions and enzymatic properties were studied primarily. The strain NJ64 was determined as genus Pseudoalteromonas sp.. The optimum cellulase-producing conditions were obtained as follows:cultural time was 72 h,initial pH value was 7. 5, cultural temperature was 10℃. The enzymatic activity of cold-adapted cellulase was the highest with pH value of about 9.0,temperature of 40℃.