优异杂交水稻亲本灌浆期种子内生细菌多样性研究

摘要

Diversity of the endophytic bacterial community structure in seeds of three parents of hybrid rice "Shen 08S","He 620S",and "16A007" in the milk was studied through conventional microbial cultivation methods.The experiment showed that the diversity of the bacterial community structure was still existed in the parental seeds of hybrid rice in the milk.The endophytic bacterial community in "Shen 08S" contained 6OTUs,and the first dominant bacterium genus was Pantoea with abundance at 52.04％,the second and third dominant bacterium genera were Pseudomonas and Rhizobium respectively;The endophytic bacterial community in "He 620S" contained 10 OTUs,and the abundance of the first dominant bacterium Pantoea was 69.02％,the second one was Pseudomonas,the third ones were juxtapositionally Rhizobium and Sphingomonas.The endophytic bacterial community in "16A007" contained 11 OTUs,and the abundance of the first dominant bacterium Pseucomonas was 45.12％,the second and third ones were Pantoea and Sphingomonas.From this study,it thus clear that the rice seed of "Shen 08S" and "He 620S" that having genetic pertinence had the same endophytic bacteria in the first and the second dominant bacterial genera;At the same time,all of three parent seeds of the rice had Pantoea and Pseudomonas,indicated that the genotype of the rice seed had a certain effect on endophytic bacterial community structure.%通过传统微生物培养方法,对处于灌浆期的杂交水稻亲本“深08S”、“和620S”及“16A007”种子内生细菌群落结构多样性进行研究.实验表明,处于灌浆期的杂交水稻亲本种子仍然存在内生细菌群落结构的多样性.“深08S”种子内生细菌含6个OTU,第一优势菌属为Pantoea,丰度为52.04％,第二和第三优势菌属分别为Pseudomonas和Rhizobium;“和620S”种子内生细菌含10个OTU,第一优势菌属为Pantoea,丰度为69.02％,第二优势菌属为Pseudomonas,并列第三优势菌属为Rhizobium和Sphingomonas;“16 A007”种子内生细菌含11个OTU,第一优势菌属为Pseudomonas,丰度为45.12％,第二优势菌属和第三优势菌属分别为Pantoea和Sphingomonas.由研究结果可见,具有遗传相关性的水稻种子“深08S”和“和620S”内生细菌的第一和第二优势菌属相同,同时,三种水稻亲本种子优势菌属都有Pantoea和Pseudomonas,表明水稻种子基因型对其内生细菌结构多样性具有一定的影响.