重组杆状病毒介导的DNMT1 siRNA对人胰腺癌细胞增殖影响的研究

摘要

Objective:This study is to observe the effects of DNMT1 siRNA recombinant baculovirus on pancreatic cancer cell.Methods:Cultured Human pancreatic cancer cell and divided into five groups: control group, negative control group, r-Bac-si-DNMT1-D1 group (D1 group), r-Bac-si-DNMT1-D2 group (D2 group), r-Bac-si-DNMT1-D3 group (D3 group).Control group were cultured only with 10% FBS and DMEM.At 24h after plating with FBS and DMEM, Negative control group, D1 group, D2 group,D3 group were transfected with corresponding baculovirus-mediated RNA interference of DNMT1(150μl/well).And after 72h transfection, use MTT to test the change of cell proliferation.Results:The cell survival rate of DNMT1 siRNA recombinant baculovirus group were significant lower than that in control group and negative control group (P<0.05).Conclusion:DNMT1 siRNA recombinant baculovirus could deduce proliferation on human pancreatic cancer by lower the cell survival rate.%目的:研究利用重组杆状病毒介导的DNMT1 siRNA对人胰腺癌细胞增殖的影响.方法:培养人胰腺癌细胞并分组如下:空白对照组、阴性对照组、病毒r-Bac-si-DNMT1-D1组(D1组)、r-Bac-si-DNMT1-D2组(D2组)、r-Bac-si-DNMT1-D3组(D3组).空白对照组仅加10%胎牛血清和DMEM培养基培养,阴性对照组、D1组、D2组、D3组加血清和DMEM培养基培养24h后依次加入空白载体r-Bac-CMV-EGFP和r-Bac-si-DNMT1-D1病毒、r-Bac-si-DNMT1-D2病毒、r-Bac-si-DNMT1-D3病毒150μl/孔,72h后分别收集各组细胞用MTT法测肿瘤细胞增殖的变化.结果:重组杆状病毒介导的DNMT1 siRNA感染的细胞存活率较空白对照组和阴性对照组明显降低(P<0.05).结论:杆状病毒介导的DNMT1 siRNA感染人胰腺癌细胞后能通过降低细胞存活率,从而影响胰腺癌细胞的增殖.