小柴胡汤对大鼠子宫内膜异位症COX-2、P450arom表达的影响

摘要

目的 探讨小柴胡汤对大鼠子宫内膜异位症(内异症)的治疗作用及其机制.方法 建立大鼠内异症动物模型,随机分为空白对照组、模型对照组、小柴胡汤组、丹那唑组、联合用药组(小柴胡汤+丹那唑),利用免疫组化方法,观察分析各组大鼠在位、异位内膜中COX一2、P450arom的表达水平.结果 COX-2、P450arom在正常子宫内膜无表达,而在内异症内膜有强表达;小柴胡汤组和联合用药组COX-2、P450arom的表达水平明显低于模型对照组(P<0.05);而西药丹那唑组COX-2的表达水平无明显变化,但P450arom的表达明显低于模型组(P<0.01).结论 小柴胡汤可能通过下调异位内膜COX-2、P450arom的表达,抑制局部雌激素的产生而达到治疗目的.%Objective To investigate the effect of Xiaochaihu soup on treatment endometriosis in rats and to explore the mechanism of Xiao chaihu soup on treatment.Methods The rat model of endometriosis was established and the rats were treated with Xiaochaihu soup.The height of endometrial epithelium on endometriotic tissue( EOS) was investigated by the morphometry method.Expression of cyclooxygenase - 2( COX - 2) and aromatase cytochrome P450 ( P450arom) in each group of endometrium and endometriotic tissue was observed by immunohistochemistry method.Results The expression of COX - 2 and P450 arom in the endometriotic tissue was lower than enilometrium in Xiaochaihu soup group and Xiaochaihu soup plus Danazol group.The expression of COX - 2 in FOS of Danazul group was of no difference with the control group although the expression of P450 arom was lower than the control group( P ＜ 0.01).Conclusion The mechanism of Xiao Chaihu Tang on the treatment endometriosis may be decreasing the expression of COX -2 and P450arom and reducing the form of estradiol.