HLX基因的表达与急性髓系白血病相关性

摘要

目的 研究HLX表达水平与AML的临床变量及AML不同危险度分层的相关性.方法 应用实时荧光定量PCR方法检测32例初治AML患者骨髓单个核细胞中HLX表达量,分析其与患者FAB分型、血象、骨髓原始细胞、分化程度及AML不同危险度分层关系.结果 初治AML患者HLX表达量在AML-M3中明显降低(P＜0.01),AML-M5中明显升高(P＜0.05);HLX表达水明与骨髓原始细胞数呈负相关(P＜0.01),与外周血血小板水平呈正相关(P＜0.05),且AML-M3HLX表达量与外周血白细胞水平呈正相关(P＜0.05),AML-M5 HLX表达量与外周血白细胞水平呈负相关(P ＜0.05);AML-M5组中分化不良组HLX表达水平明显高于分化良好组,(P＜0.05),病例组及非M3组初治AML患者中,低危组与中危组HLX表达水平差异无统计学意义(P＞0.05).结论 HLX表达水平在部分亚型(如AML-M5)中表达升高,且在不同的FAB分型中有差异.该基因与白血病细胞的增殖、分化和成熟相关.本研究暂无证据表明HLX在不同AML危险度分层中有差异,仍需进一步实验研究.%Objective To investigte the correlation between HLX expression level and AML's clinical variables and different risk stratification.Methods A rcal-time quantitative PCR was used to detect HLX expression in the bone marrow mononuclear cells of 32 de novo AML patients with initial treatment.The relationship between HLX expression level and clinical parameters (FAB classification,hemogram,bone marrow progenitor cells,differentiation and different risk stratification) was investigted.Results The HLX expression of patients with AML was significantly decreased in AML-M3 (P ＜ 0.01),and increased in AML-M5 (P ＜ 0.05).It was negatively correlated with bone marrow progenitor cells (P ＜ 0.01),and was positively correlated with the number of peripheral blood platelet (P ＜0.05).The expression of HLX in AML-M3 was positively correlated with peripheral blood leukocyte levels (P ＜ 0.05),and it was negatively correlated with leukocyte cell level in peripheral blood in AML-M5 (P ＜ 0.05).In AML-Ms groups,the expression levels of HLX of poorly differentiated group were significantly higher than those of the well-differentiated groups (P ＜ 0.05).In AML patients with initial treatment in patient group and non-M3 group,there was no significant difference between the low-risk group and the central risk group (P ＞0.05).Conclution HLX expression levels were elevated in some subtypes,such as AML-M5.and there were differences in different FAB types.HLX gene is associated with the proliferation,differentiation and maturation of leukemia cells.However there was no evidence that HLX is different in different AML risk stratification,which needs further experimental studies.