松果菊苷促进卵巢癌模型裸鼠存活、降低肿瘤增殖和转移

摘要

探究松果菊苷 (echinacoside, ES) 对人卵巢癌模型裸鼠的存活、肿瘤生长和迁移能力的影响.方法 将60只裸鼠随机分为4组, 即皮下注射稳定表达荧光素酶的卵巢癌CoC1细胞、用等量生理盐水处理的阴性对照组 (Control, Ctrl)、不同剂量ES (20、 50、 100 mg/kg) 处理组. Ctrl组每天注射等体积的生理盐水, ES处理组每天分别腹腔注射20、 50、 100 mg/kg的ES, 连续处理25 d, 每天记录裸鼠存活情况和肿瘤体积; 处理第26天, 处死小鼠, 取瘤组织进行免疫组化以检测Ki67和血管内皮细胞生长因子(vascular endothelial groeth factor, VEGF) 的表达; TUNEL检测细胞凋亡.结果 与Ctrl组比较, ES (20、50、 100 mg/kg) 组裸鼠存活率明显升高 (P<0.05); 肿瘤体积显著降低, 移植瘤组织中Ki67阳性细胞明显减少 (P<0.05); ES (50、 100 mg/kg) 组肿瘤组织凋亡细胞与Ctrl组比较明显增多 (P<0.05); ES (20、 50、 100 mg/kg) 组VEGF阳性细胞数明显低于Ctrl组 (P<0.05), 此外, ES (50、 100 mg/kg) 组小鼠心脏、胰腺、肝脏和肾脏的荧光强度与Ctrl组比较明显降低.结论 ES能促进卵巢癌模型裸鼠的存活,降低肿瘤的生长, 还能抑制卵巢癌肿瘤转移.%Objective To investigate the effects of echinacoside ( ES) on the survival of the mouse model of human ovarian cancer, and the growth and metastasis of tumors.Methods Sixty nude mice were randomly divided into four groups.In the control group, the mice were subcutane-ously injected with ovarian cancer CoC1 cells that could stably express luciferase and then treated with equal amount of saline.In ES groups ( 20, 50, 100 mg/kg) , different doses of ES ( 20, 50, 100 mg/kg) were intraperitoneally given to the mouse model of ovarian cancer.The treatment lasted for 25 days.The survival and tumor volume of nude mice were recorded every day.The mice were sacrificed at 26 days.Tumor tissues were harvested and immunohistochemically detected for the expression levels of Ki67 and vascular endothelial growth factor ( VEGF) .TUNEL was used to de-tect apoptosis.Results Compared with the control group, the survival rate of ES (20, 50, 100 mg/kg) groups was significantly increased (P<0.05), the tumor volume significantly decreased, and the Ki67 positive cells in the transplanted tumor tissues significantly decreased ( P <0.05) . .The number of apoptotic cells in the ES (50, 100 mg/kg) groups was significantly higher than that in the control group ( P <0.05) .The number of VEGF positive cells in the ES (20, 50, 100 mg/kg) group was significantly lower than that in the control group (P<0.05) .In addition, the fluorescence intensity of the heart, pancreas, liver and kidney of the ES (50, 100 mg/kg) groups was significantly lower than that of the control group.Conclusion ES promotes the survival of the mouse model of ovarian cancer, and inhibits the growth and metastasis of ovarian cancer.