Objective To determine the effect of doxycycline in different concentrations on the expression of Cx26/Cx32 proteins and their function in gap junction intercellular communication in HeLa cervical cancer cells. Methods Western blotting was used to detect Cx26/Cx32 protein in HeLa cells. Fluorescence trace was used to assay the gap junction intercellular communication mediated by Cx26/Cx32 in HeLa cells. Results The expression of Cx26/Cx32 was increased with the doxycycline concentration from 0. 01 to 1μg/ml, as determined by western blotting. The fluorescence trace was enhanced with the increased concentration of doxycycline. Conclusion The cellular models of different gap junction intercellular communication were preparable by treatment with different concentrations of doxycycline.%目的 在HeLa宫颈癌细胞中研究不同浓度的多西环素对缝隙连接蛋白Cx26/Cx32表达及由其形成的缝隙连接通讯功能的影响.方法 采用Western印迹检测HeLa细胞中Cx26/Cx32的蛋白表达;荧光示踪实验用于检测HeLa细胞中由Cx26/Cx32形成的缝隙连接通讯功能.结果 Western印迹结果显示多西环素在0.01～1 μg/ml的范围内,随着剂量的增加,Cx26/Cx32蛋白表达水平增加;荧光示踪实验结果显示HeLa细胞之间的荧光传递随着多西环素增加也相应增强.结论 采用加入不同浓度多西环素的方法,可制备缝隙连接通讯功能强弱不同的细胞模型.
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