Effect of Deoxypodophyllotoxin Isolated from Anthriscus sylvestris Roots on the Expression of Cell Cycle-regulatory Proteins in HeLa Cells

摘要

Deoxypodophyllotoxin (DPPT), a natural flavolignan, exhibits anti-inflammatory and anti-tumor activities. However, the molecular mechanism of DPPT underlying the modulation of cell cycle progression is currently unknown. In this study, we determined the effect of DPPT isolated from Anthriscus sylvestris roots on the expression of cell cycle-regulatory proteins. We observed that DPPT treatment induced the arrest of cell cycle at the G2/M phase in HeLa cervix carcinoma cells. Immunoblot experiments demonstrated that DPPT causes the phosphorylation of ERK MAPK and p21~(Wan/Cipl) as well as up-regulation of PLK1 and cyclinBl. This is accompanied by the activation of Cdc25C and Cdkl. These data suggest that DPPT may have a potential effect in the cell cycle arrest at the G2/M phase through the activation of the Cdkl/cyclin Bl complex through Cdc25C.