壳寡糖预处理对大鼠肠缺血再灌注损伤回肠NF-κB表达的影响

摘要

目的：：研究壳寡糖预处理对大鼠肠缺血再灌注损伤(I/R)回肠核转录因子κB(NF-κB)表达的影响。方法：某院于2015年2月~2015年7月间将实验室饲养的40只健康雄性 SD 大鼠随机分为 I/R 组、壳寡糖预处理组两组,每组各20只。I/R 组术前60min 生理盐水灌胃,并通过手术建立肠 I/R 模型；壳寡糖预处理组在肠 I/R 模型建立前5d 按1500mg/kg 给予壳寡糖灌胃预处理,连续5d,并于建模前30min 再灌胃1次,建立 I/R 模型。灌注处死大鼠后通过病理组织切片观察和免疫组化分析,观察两组肠黏膜损伤程度和 NF-κB 检测表达。结果：壳寡糖预处理肠黏膜损伤 Chiu(2.64±0.11)分、NF-κB 蛋白表达(0.17±0.01)、NF-κB mRNA(0.57±0.05)较 I/R 组(4.18±0.14)分、(0.23±0.03)、(0.70±0.05)均较低,相较差异显著(P <0.05)。结论：壳寡糖能较好的抑制 NF-κB表达,对减轻回肠缺血再灌注黏膜损伤效果显著。%Objective:To study the effects of pretreatment with chitosan oligosaccharide on the expression of nuclear factor kappa B (NF-κB)in ileum of rats with intestinal ischemia-reperfusion injury (I/R).Methods:40 healthy male SD rats which were fed in the laboratory between February 2015 and July 2015 were random-ly divided into I/R group and chitosan oligosaccharide pretreatment group with 20 rats in each group;I/R group were treated by intragastric administration of normal saline at 60min before operation and the intestinal I/R model was established by operation;Chitosan oligosaccharide pretreatment group were treated by pre-treatment with intragastric administration of 1500mg/kg chitosan oligosaccharide at 5d before establishment of intestinal I/R model and the treatment lasted for 5d,with intragastric administration again at 30min be-fore modeling.Then,I/R model was established.After lethal perfusion,the rats were sacrificed by patholog-ical biopsy and immunohistochemical analysis,The degree of intestinal mucosal damage and the expression of NF-κB in the 2 groups were observed.Results:Chitosan oligosaccharide pre processing intestinal mucosa Chiu (2.64±0.1 1),NF kappa B protein expression (0.17 ±0.01 ),NF kappa B mRNA (0.57 ±0.05 )compared with I / R group (4.18±0.14),(0.23±0.03),(0.70±0.05)were lower,with statistically significant differ-ence (P <0.05).Conclusion:Chitosan oligosaccharide could inhibit the expression of NF-κB,and significant-ly reduce the effect of mucosal injury in the intestinal ischemia-reperfusion injury.