多发性骨髓瘤患者p15、p16基因甲基化及其与预后的相关性

摘要

Objective To illustrate the role of hypermethylation of p15 and p16 genes in the pathogenesis and prognosis of multiple myeloma(MM).MethodsForty-seven patients with multiple myeloma were tested for methylation status of p15,p16 genes by nested-methylation specific PCR(n-MSP),and the clinical data and prognostic factors of the 47 patients were analyzed.Results Twenty-eight (59.57%)cases of methylation of p15 gene were observed,and twenty-seven(57.45%)cases of methylation of p16 gene were observed.p15.p16 genes methylations were frequently detected in cases of stageⅡand ⅢMM than stage Ⅰ MM.Conclusion There Was a strong correlation between methylation of p15 and p16 gene.which might be related with the pathogenesis and prognosis of MM.%目的 探讨p15、p16基因的高甲基化与多发性骨髓瘤(MM)的发病和预后之间的关系.方法 采用巢式甲基特异性PCR法(nMSP)检测47例MM患者p15、p16基因的甲基化状态,并对患者的临床资料及预后因素进行分析.结果47例MM患者p15、p16基因的甲基化比例分别为59.57%(28/47)、57.45%(27/47).两者同时存在甲基化者23例(48.94%).Ⅱ、Ⅲ期MM患者p15、p16基因甲基化率明显高于Ⅰ期患者.结论 p16、p15基因的高甲基化与MM的发病及预后相关.