乙酰肝素酶及其相关因子mRNA在白血病细胞中的表达

摘要

目的 观察乙酰肝素酶(HPA)及其相关因子mRNA在白血病细胞中的表达,研究白血病细胞表面止血平衡改变.方法 选取白血病患者40例(研究组),缺铁性贫血患者20例(对照组).采用半定量反转录聚合酶链反应(RT-PCR)方法测定受试者骨髓单个核细胞组织因子(TF)、尿激酶型纤溶酶原激活物受体(uPAR)、HPA mRNA的含量.结果 研究组骨髓中单个核细胞TF、uPAR、HPAmRNA的表达与对照组相比均升高(均P＜0.05),35例初发白血病患者中急性髓系白血病(AML)组(23例)骨髓中单个核细胞TF、uPAR、HPA mRNA高于慢性粒细胞白血病(CML)组(4例)、慢性淋巴细胞白血病(CLL)组(3例)、急性淋巴细胞白血病(ALL)组(5例)(对照组、AML组、CML组、ALL组、CLL组TF分别为0.66±0.03、2.56±0.37、1.33±0.06、1.93±0.08、1.23±0.07,uPAR分别为0.53士0.02、1.89±0.26、0.92±0.15、1.44±0.01、1.19±0.16,HPA分别为2.37±0.24、1.42±0.13、1.68±0.09、1.54±0.12,均P＜0.05).结论 白血病细胞中HPA、TF、uPAR mRNA表达增高;不同类型白血病表达水平不同.提示TF、uPAR、HPA可能影响白血病细胞表面止血平衡,不同类型白血病细胞表面止血平衡改变不同.%Objective To explore whether the expression of heparanase (HPA) and other coagulation proteins on blast membrane could determine the hemostatic balance on the surface of leukemia cells.Methods 40 patients with leukemia (study group) and 20 patients with iron deficiency anemia (control group) were analyzed,the levels of TF,uPAR,HPA mRNA were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).Results The levels of TF,uPAR,HPA mRNA in study group were higher than those in the control group (P ＜ 0.05).The levels of TF,uPAR,HPA mRNA in acute myeloid leukemia (AML) group (23 cases) were significantly higher than those in the acute lymphoblastic leukemia (ALL) (5 cases),chronic myelocytic leukemia (CML) (4 cases),chronic lymphocytic leukemia (CLL) groups (3 cases) (in control,AML,CML,ALL,CLL groups,TF levels were 0.66±0.03,2.56±0.37,1.33±0.06,1.93± 0.08,1.23±0.07,uPAR were 0.53±0.02,1.89±0.26,0.92±0.15,1.44±0.01,1.19±0.16,HPA were 2.37±0.24,1.42±0.13,1.68±0.09,1.54±0.12,respectively,all P ＜ 0.05).Conclusions The TF,uPAR and HPA predominate in leukemia cells.The expression of coagulation proteins on blast membrane may determine the hemostatic balance on the surface of leukemia cells,and the hemostatic balance on the blast surface may contribute to thombohemorrhagic syndrome manifestations in leukemic patients.