METHOD FOR EFFECTIVE INDUCTION OF APOPTOSIS IN SUSPENSION CULTURE OF TUMOR BONE MARROW CELLS Ph⁺ AND LEUKEMIC PATIENT PERIPHERAL BLOOD USING ANTI-SENSE OLIGONUCLEOTIDES SPECIFIC TO MRNA OF GENES INHIBITING APOPTOSIS

摘要

FIELD: molecular biology, medicine, oncology.;SUBSTANCE: invention relates to development of methods for specific biotherapy of leucosis Ph⁺. Method involves treatment of suspension cultures of cells Ph⁺ with anti-sense oligonucleotides of length 16-26 nucleotide links that are specific to mRNA of oncogen BCR/ABL in combination with anti-sense oligonucleotides to mRNA of other genes blocking apoptosis of cells Ph⁺ distinctive from BCR/ABL. Invention provides the development of the experimental basis for carrying out oligonucleotide genetic therapy of leucosis Ph⁺.;EFFECT: improved induction method.;5 dwg, 4 ex