Objective To investigate the effect of dl-3n-butylphthalide on the expression of the serum neuron specific enolase (NSE) and S-100/3 protein in the rat model of cerebral ischemia reperfusion. Methods 198 healthy male Wistar rats were chosen to establish cerebral ischemia reperfusion model with middle cerebral artery thread embolism method and were randomly divided into sham operation group, model group, and ischemia and reperfusion treated with dl-3n-butylphthalide group. The last two groups were divided into five subgroups according to reperfusion time interval of 6, 24, 48, 72 and 168 h after brain ischemia. There were 18 rats in each subgroup and in the sham operation group. After the success of cerebral ischemia model in 1 h, the treatment groups were given dl-3n-butylphthalide at dose of 1mL/(100 g·d). The other groups were given the same dose of consumption of peanut oil. The neurological deficit scores were conducted for rats in each group at different time point, and the infarct sizes were measured with 2,3,5-triphenyltetrazolium chloride staining. The number of apoptotic cells were detected by TdT mediated dUTP nick end labeling method, and neuron specific enolase and S-100β protein concentration in serum were measured by enzyme-linked immunosorbent assay. Results The serum NSE and S-100β protein concentration in treatment group and model group had increased in the ischemia reperfusion to different degrees and reached the highest in the onset of 48 and 72 h in two groups. Compared with model group, the expression level of serum NSE and S-100β protein concentration, neurological deficit scores, infarct sizes, apoptotic cells of the treatment group decreased significantly and the difference had statistical significance (P <0.05). Conclusion Dl-3n-butylphthalide can effectively reduce the serum NSE and S-100β protein concentrations of ischemia reperfusion in rat brain, and improve the symptoms of neurological deficit, which may play some protective role on cerebral ischemia reperfusion injury.%目的 探讨丁苯酞对大鼠脑缺血再灌注损伤后血清神经元特异性烯醇化酶(NSE)和S-100β蛋白表达水平的影响.方法 健康雄性Wistar大鼠198只随机分为假手术组、模型组和丁苯酞治疗组,后两组再分为脑缺血2h后再灌注6、24、48、72和168 h亚组,每亚组及假手术组各18只.应用线栓法建立大鼠大脑中动脉阻塞再灌注模型,造模成功治疗组于脑缺血1h后给予丁苯酞1 mL/100 (g·d)灌胃,其他各组均给予等量花生油灌胃.对各组大鼠在不同时间点分别进行神经功能缺损评分、红四氮唑染色测量脑梗死体积、原位末端标记法检测凋亡细胞数目,并采用酶联免疫吸附法对不同时间点各组大鼠血清NSE和S-100β蛋白浓度进行动态检测.结果 治疗组与模型组NSE和S-100β蛋白浓度在脑缺血再灌注损伤后均有不同程度升高,分别在起病48和72 h达高峰,且治疗组NSE、S-100β蛋白浓度、神经功能缺损评分、脑梗死体积、凋亡细胞数目均低于同一时间点模型组,差异均有统计学意义(P＜0.05).结论 丁苯酞可有效降低脑缺血再灌注损伤后血清NSE和S-100β蛋白浓度,改善神经功能缺损症状,对脑缺血再灌注损伤有一定的保护作用.
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