Let-7在正常乳腺组织和乳腺癌组织中的表达

摘要

目的 研究Let-7基因在乳腺癌中的表达特征.方法 随机抽取28例乳腺癌患者收集其癌组织和癌旁正常组织,利用TRIzol提取总RNA,实时荧光定量PCR检测基因表达量.结果 Let-7前体在癌组织中的相对表达量(9.65±2.31),低于癌旁正常组织(10.0±52.81),P=0.048,而Let-7基因则无显著变化.前体Let-7与最长行经(日)之间有依存关系(b=0.875,P=0.020);初潮年龄与前体Let-7的表达在癌组织中的呈负相关(r=-0.484,P=0.049),在正常组织中呈正相关(r=0.502,P=0.048).结论 Let-7前体在癌组织中的表达低于正常组织,最长行经天数越长则Let-7表达越高.%Objective To study the expression characteristics of Let-7 genes in breast cancer.Methods Twenty-eight patients with breast cancer were randomly selected,and their cancer tissue and adjacent normal tissue were collected.TRIzol was used to extract the total RNA and real-time quantitative PCR was used to detect the relative gene expression levels.Results The expression of precursor Let-7 in cancer tissue was (9.65 ± 2.31),which was lower than that in normal tissue (10.05 ± 2.81),P =0.048.Precursor Let-7 had dependence relationship with the long menstruation (b =0.816,P =0.029).The menarche age showed a positive correlation with precursor Let-7 in normal tissue (r =0.502,P =0.048) and a negative correlation in cancer tissue (r =-0.484,P =0.049) Conclusions The expression of precursor Let-7 in cancer tissue is lower than that in normal tissue.The period of menstruation is a protective factor to breast cancer.