This study aims to get high expression level of GLP-1 derivate (GP62) in high cell-density recombinant Escherichia coli BL21 ( DE3 ) by optimizing its fed-batch culture medium. The effects of different basal medium, yeast extract and inorganic salt concentration on cell growth and expression of GLP-1 deftvate (GP62) in recombinant E. coli BL21 (DE3) were investigated by single factor test and orthogonal array. Optimized 2YT-GP62 medium was obtained by improving the 2YT medium. The highest productivity of 4. 40 mg fusion protein per gram of dry cells and the final cell density of 21.3 ( OD600 ) were obtained in 5L fermentor induced by 0. 5 mmol/L IPTG. The final cell density was 4. 4 times of that in LB culture medium and the expression level of GP62 was improved from 27. 5％ to 31.2％. The results show that 2YT-GP62 medium can be used for batch cultivation for production of recombinant GLP-1 derivate.%对重组E.coli BL21 (DE3)高密度表达胰高血糖素样肽-1衍生物(GP62)的发酵培养基进行了优化.通过单因素实验与正交实验相结合的方法,依次对基础培养基,发酵培养基中碳源、氮源、无机盐等成份进行优化.在2YT培养基基础上,获得优化的发酵培养基2YT-GP62.在5L全自动发酵罐中利用终浓度0.5 mmol/L IPTG诱导,可获得的细胞密度为细胞干质量6.82g/L发酵液(OD600=21.3),为LB培养基中的4.4倍,目的蛋白表达率由27.5%上升到31.2%,融合蛋白得率为4.40 mg/g干细胞.结果证明优化后的发酵培养基可用于重组GLP-1衍生物分批发酵生产.
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