目的:探讨hTERC基因检测在宫颈上皮内瘤变差异化治疗的应用.方法:利用荧光原位杂交技术(FISH)观察人类染色体端粒酶基因(hTERC)扩增程度与宫颈上皮内瘤变(CIN)程度关系.检测63例宫颈液基薄层细胞涂片(TCT)和50例宫颈活检石蜡切片hTERC基因的扩增程度.结果:两组结果均显示,随着TBS分级或病理分级的升高,hTERC基因阳性率增加.hTERC基因异常细胞比例与TBS分级或病理分级呈正相关,等级相关系数为0.403/0.612.结论:hTERC基因扩增程度可能为宫颈疾病发展的重要风险预测因子,反映宫颈上皮内瘤变的病变程度,可辅助病理分级的准确判断.%Aim;To observe the association between the human telomerase RNA component (hTERC) amplification degree and cervical intraepithelial neoplasia with the technology of fluorescence in situ hybridization (FISH) and discuss the application of hTERC genetic testing in cervical intraepithelial neoplasia. Methods; Respectively, 63 cases of cervical liquid-based cell and 50 cases of cervical bioptic paraffin sections were detected the hTERC gene amplification by FISH, the positive rate of hTERC gene amplification increased with increasing TBS classification or pathologic grading. Results; In the both group, it was positively correlated between the proportion of the hTERC abnormal cells and the grade of cervical intraepithelial neoplasia (P <0. 05). The correlation coefficient was 0.413/0. 612. Conclusion: It' s reflected that the extent of cervical intraepithelial neoplasia lesions, assisted to judge the pathological grade accurately, determined further to guide the clinical treatment of cervical precancerous lesions program formulation.
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