三仁汤干预脾胃湿热型慢性糜烂性胃炎后HSP-70及IL-1β的变化

摘要

Aim:The mechanisms of San Ren decoction interfering with chronic erosive gastritis were discussed by observing the TCM syndrome and the changes of HSP70 and IL-1β.Methods:One hundred and forty CEG patients were divided into two groups randomly.Each group was administrated with San Ren decoction and placebo for 4 weeks respectively.The changes of clinical manifestations were documented before and after treatment.The expression of HSPT0 and IL-1β were assayed by Real-time PCR.Results:The total effect rate of San Ren decoction in treating CEG is 84.28％.The total effect rate in Control group is 30％.Before treatment,the expression of HSP70 mRNA is on the decline,whereas the expression of IL-1β mRNA is on the increment.After treatment,the expression of HSP70 mRNA in intervention group is on the increment.However the expression of IL-1β mRNA in intervention group is on the decline.There is a significant difference in the expression of HSP70 and IL-1β before and after treatment in intervention group (P ＜ 0.05).There is no significant difference in the expression of HSP70 and IL-1β mRNA in control group before and after treatment(P ＞ 0.05).Conclusion:San Ren decoction exerts an exact effect on alleviating chronic erosive gastritis by regulating the expression of HSP70 and IL-1β.%目的:基于治疗前后热休克蛋白70(HSP-70)、白细胞介素-1β(IL-1β)以及中医证侯变化,主客观指标合参,评价三仁汤干预脾胃湿热型慢性糜烂性胃炎(CEG)的临床疗效及可能机制.方法:140例符合诊断标准、纳入标准的CEG患者按1∶ 1随机分为治疗组和对照组,治疗组予三仁汤干预4周,对照组予安慰剂干预4周.详细记录治疗前、治疗后中医证侯变化；应用实时免疫酶链反应(Real-time PCR)技术检测胃黏膜HSP-70、IL-1β变化.结果:三仁汤治疗脾胃湿热型慢性糜烂性胃炎总有效率为84.28％,对照组总有效率为30％,治疗前两组患者HSP70 mRNA表达呈下降趋势,IL-1βmRNA表达呈上升趋势；治疗后,治疗组血清HSPT0 mRNA表达呈上升趋势,与治疗前比较有统计学差异(P ＜0.05)；IL-1βmRNA变化较治疗前比较,变化明显下降,有统计学差异(P＜0.05)；对照组血清HSP70及IL-1βmRNA较治疗前比较,无统计学差异(P＞0.05).结论:三仁汤可能通过诱导HSP-70表达,抑制IL-1β分泌从而发挥治疗慢性糜烂性胃炎作用.