益气温阳对脓毒症内质网应激途径淋巴细胞凋亡的作用

摘要

Aim:To study the effect of Fuzheng method on lymphocyte apoptosis in the Endoplasmic Reticulum Stress pathway of sepsis.Methods: The animal model of sepsis was established by cecal ligation and puncture(CLP),and the clinical equivalent dose of Shenfu injection and Shenmai injection were given respectively.The apoptosis rate was detected by Annexin V-FITC/PI double staining method, the pathological changes of spleen tissue were observed by HE staining,and the expression of GRP78 and CHOP protein and mRNA in the endoplasmic reticulum stress pathway of spleen cells were detected. Results:1.The apoptosis rate of spleen cell in model group was significantly higher than that in other groups(P