Aim:To determine the levels of 5-hydroxytryptamine (5-HT), histamine (HA) and prostaglandin E2 (PGE2) using different methods in order to compare the analgesic effects among fireneedle, catgut implantation and acupuncture and to explore the chronic pain-relieving mechanism for the treatment of rheumatoid arthritis by fire needle.Methods: A total of 50 Sprague Dawley rats were randomly divided into a normal group (n=10), model group (n=10), fire needling group (n=10), acupuncture group (n=10) and catgut implantating group (n=10).Complete Freund's adjuvant (CFA) was injected into the plantar area of the right hindpaw of the rats to make the model of rheumatoid arthritis.In each group, "Yanglingquan" (ST 36) and "Kunlun" (BL 60) were selected as the major points for fire needling, acupunture and catgut implantation.The levels of 5-HT, HA and PGE2 were measured by Mircodialysis and HPLC-UV.Results: After modeling, the levels of 5-HT, HA and PGE2 in each group were significantly higher than the normal group (P<0.01).After the treatment, the levels of 5-HT, HA and PGE2 were found decreased in each group in comparison with the untreated group.5-HT, HA and PGE2 of fireneedle group was found to be at low levels in 8 hours and there were significant differences compared with the control groups (P<0.01).The levels of 5-HT, HA and PGE2 of acupunture group were at low level during 2 hours.The levels of 5-HT, HA and PGE2 of catgut implantation group decreased in 2 or 4 hours, which were significanth different from those of the model control groups (P<0.05) but no difference in the early treatment (P>0.05).Conclusion:Fire needle, acuputure and catgut implantation are all effective on decreasie of 5-HT, HA and PGE2 levels.Compared with acuputure and catgut implantation groups, fire needle has more pronounced analgesic effect and better therapeutic efficacy by reducing the levels of the neurotransmitters and serum inflammatory factors, and thus relieving the pain of Adjuvant arthritis.The first instant analgesia duration of fire needle is longer than the others, while the analgesic effects of catgut implantation appeared in more than 2 hours.%目的: 观察不同干预方法对风湿性关节炎模型大鼠病变组织中5-羟色胺(5-HT)、组胺(HA)和前列腺素E2(PGE2)含量影响与镇痛效应,探讨火针缓解类风湿性关节炎慢性疼痛的作用机制.方法:将健康雄性SD大鼠50只,随机分为正常对照组、模型组、针刺组、穴位埋线组和火针组,各10只,以完全弗氏佐剂(CFA)注入大鼠右后足垫皮肤内制作类风湿性关节炎模型,3组均取右侧"昆仑"、"阳陵泉"作为治疗穴位,分别行火针、普通针刺、穴位埋线治疗,利用微透析采样技术(MD)在线提取大鼠局部组织液,结合HPLC-UV检测技术,监测类风湿性关节炎模型大鼠不同时段内局部病变组织5-HT、HA和PGE2含量的变化.结果: 造模后大鼠肌肉组织中5-HT、HA和PGE2含量较正常组明显增高,热刺痛爪退缩阈值降低(P<0.01).治疗后,各组大鼠TWL阈值升高,病变肌肉组织内5-HT、HA、PGE2含量较治疗前均有不同程度下降,火针组5-HT、HA、PGE2含量分别在2、4、8 h处于低水平状态,与模型组比较有显著性差异(P<0.01);普通针刺组5-HT、HA、PGE2含量分别在1、2 h处于低水平状态;穴位埋线在治疗前期与模型组无统计学差异(P>0.05),该组下降时间点分别于治疗后2、4 h,与模型组有统计学差异(P<0.05).结论: 火针、普通针刺、穴位埋线均可以降低局部肌肉组织内5-HT、HA和PGE2含量;火针镇痛效应优于普通针刺组和穴位埋线组,且火针首次即时镇痛维持时间长于普通针刺组和穴位埋线组,穴位埋线则至少需要2 h以上才能发挥镇痛效应.
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