顶空气相色谱法测定人参根皂苷中的大孔吸附树脂残留物

摘要

To establish headspace-GC method for the determination of residua of macroporous resin in the saponins extracted from the roots of Panax ginseng C A Meyer, the chromatographic separation was performed.The column temperature was programed, with an initial temperature of 60 ℃ (hold 22 min), at an increasing rate of 50 ℃/min to 200 ℃ (hold 6 min); the injector temperature was set at 220 ℃ and FID detector temperature was set at 260 ℃; solvent was 40％ N, N-Dimethyl acetamide ( DMF ); the carrier gas was nitrogen. All the residua including n-hexane, benzene, toluene, 1,4-xylene, styrene and 1,4-diethyl-benzene gave good linearities and the recoveries.%采用顶空气相色谱法测定人参根皂苷中大孔树脂残留物的质量浓度.色谱柱初始温度60℃,保持22 min,以50℃/min升至200 ℃,保持6 min,氢火焰离子检测器温度为260 ℃,进样口温度220 ℃;用质量分数为40%的N,N-二甲基甲酰胺作溶剂,载气为氮气.实验结果表明,正己烷、苯、甲苯、对二甲苯、苯乙烯、对二乙基苯6种大孔树脂残留物在各自的质量浓度范围内呈良好的线性关系,且回收率符合要求.