Evaluation of proliferative activities in Wilms'tumor

摘要

Objective: To assess the potential significance of proliferating cell nuclear antigen (PCNA) labelling indexes (PCNA-LI) and AgNORs number in evaluation of proliferative activity of Wilms′ tumor. Methods: A silver staining for AgNORs and an immunohistochemical method PCNA staining were performed on the biopsy specimens taken from 34 children with Wilms′ tumor. Results: No significant differences were observed between PCNA-LI and the pathological types and clinical stages, whereas fraction of S-phase and PI and the number of AgNORs were significantly higher in patients with PCNA-LI≥25% than that in patients with PCNA-LI<25%. The number of AgNORs/cell correlated well with both pathological types and clinical stages. The combination of PCNA-LI and AgNORs can accurately reflect the proliferative activity of cancer cells in Wilms′ tumor. Conclusion: The current pathological types and clinical stages may reflect the aggressive activity in Wilms′ tumor, but insufficient. The simultaneous determination of PCNA-LI and AgNORs count could be used as the essential complementarity of conventional pathological types and stages for accurate evaluation of biologic behaviour of Wilms′ tumor.%目的:评价PCNA标记指数及AgNORs计数在肾母细胞瘤增殖活性判定中的意义.方法:对34例肾母细胞瘤标本进行PCNA免疫组织化学染色及核仁组织区嗜银蛋白组织化学染色.结果:PCNA-LI与病理学分型及临床分期无关,但PCNA-LI≥25%组的S期细胞数、PI值及AgNORs数量均显著高于PCNA-LI<25%组;AgNORs计数与病理学分型及临床分期关系密切;PCNA-LI 与AgNORs计数结合可准确反映肾母细胞瘤的增殖活性.结论:目前肾母细胞瘤的病理学分型和临床分期可以反映其侵袭性,但并不充分;在准确评价肾母细胞瘤生物学特性方面,PCNA-LI 与AgNORs联合应用可以作为传统病理学分型及临床分期的必要补充.