多发性骨髓瘤和慢性淋巴细胞白血病患者血浆lncRNA MALAT1、LincRNA-p21和GAS5的表达水平及其诊断意义

摘要

目的:探讨多发性骨髓瘤(MM)和慢性淋巴细胞白血病(CLL)患者血浆长链非编码 RNA (lncRNA)MALAT1、LincRNA-p21和 GAS5相对表达水平的差异,阐明3种 lncRNA在 MM与 CLL鉴别诊断中的意义.方法:选取60例 MM患者(MM组)、60例CLL患者(CLL组)和60名健康体检者(对照组)作为研究对象,对3组研究对象血浆 lncRNA MALAT1、LincRNA-p21和 GAS5的表达水平进行检测和比较.结果:MM组患者血浆 lncRNA MALAT1和 GAS5相对表达水平明显高于其他2组(P<0.05).CLL组患者血浆 LincRNA-p21相对表达水平明显低于其他2组(P<0.05).血浆 LincRNA-p21诊断 CLL的受试者工作特征曲线(ROC)下面积(AUC)为0.850,95% 可信区间(CI)为0.780~0.921;血浆 lncRNA MALAT1和GAS5在 MM诊断中的 AUC 分别为0.898和0.815,95% CI 分别为0.836~0.959和0.740~0.890;血浆lncRNA MALAT1、LincRNA-p21和 GAS5在CLL和 MM鉴别诊断中的 AUC分别为0.878、0.778和0.805, 95% CI分别为0.814~0.942、0.691~0.865和0.727~0.882.结论:血浆 lncRNA MALAT1和 GAS5的高表达与MM的发病有关,而血浆LincRNA-p21低表达与CLL的发病有关,上述3种lncRNA的血浆相对表达水平可作为 MM和CLL诊断中的辅助指标.%Objective:To explore the differences of the relative expression levels of plasma long chain non encoding RNA(lncRNA)MALAT1,LincRNA-p21 and GAS5 in the patients with multiple myeloma(MM)and chronic lymphocytic leukemia(CLL),and to clarify their significances in the differential diagnosis of MM and CLL. Methods:A total of 60 cases of MM patients(MM group),60 cases of CLL patients(CLL roup)and 60 healthy persons after physical examinations(control group)were selected as the subjects.The plasma levels of lncRNA MALAT,LincRNA-p21 and GAS5 of the subjects in three groups were detected and compared.Results:The relative expression levels of lncRNA MALAT1 and GAS5 in plasma of the patients in MM group were significantly higher than those in the other two groups(P<0.05);the relative expression level of LincRNA-p21 in plasma of the patients in CLL group was significantly lower than those in the other two groups(P<0.05).The area under receiver operating characteristic curve(ROC)(AUC)of plasma LincRNA-p21 in the diagnosis of CLL was 0.850, its 95% confidence interval(CI)was 0.780-0.921. The AUC of plasma lncRNA MALAT1 and GAS5 in the diagnosis of MM were 0.898 and 0.815;their 95% CI were 0.836-0.959 and 0.740-0.890,respectively.The AUC of plasma lncRNA MALAT1,LincRNA-p21 and GAS5 in the differential diagnosis of CLL and MM were 0.878,0.778 and 0.805,and their 95% CI were 0.814 - 0.942,0.691 - 0.865 and 0.727 - 0.882, respectively.Conclusion:The incidence of MM is related with the high expressions of lncRNA MALAT1 and GAS5 in plasma and the incidence of CLL is related with the low expression of LincRNA-p21 in plasma. The relative expression levels of the above lncRNA can be used as the auxiliary indexes in the diagnosis of MM and CLL.