The effect of deuterium ion bombardment majority targeted to produce 14Mev fast neutron irradiation, with dosage 0.6—4.8Gy, on production of cellulose from Trichoderma viride AS3.3711 was studied. The death rate was in a rising trend when irradiation dosage was 0.6—4.8 Gy. The results indicated the death rate of spores reached 80.0 ％ when radiation dosage was 1.2 Gy. It was shown that one positive mutant had significant difference compared to the original with paired samples test (t-test). The filter paper activity of cellulase of strain Fnl0 - 1 reached 633.63 U/mL, with 4 times enhanced, compared with the initial strain. And the CMC activity of cellulase of strain Fnl0 - 1 reached 914.4 U/mL,with 30 times enhanced, compared with the initial strain.%利用快中子对绿色木霉AS3.3711进行辐照,辐照剂量为0.6～4.8 Gy.研究不同剂量快中子对绿色木霉孢子致死率和遗传稳定性的影响,得到了提高纤维素酶活性的菌株.结果表明:辐照剂量在0.6～4.8 Gy 范围内绿色木霉的致死率呈逐渐上升趋势,辐照剂量为1.2 Gy时,绿色木霉的致死率达到80.0%,此突变株的羧甲基纤维素酶活(CMC酶活)可达914.4 U/mL,比出发菌株提高了30倍,滤纸酶活(FPA酶活)为633.63 U/mL,比出发菌株提高了4倍.
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