以六年生鲜人参、生晒参、红参为试验材料,建立腺苷和L-焦谷氨酸的含量测定方法:RP-HPLC法分析样品中腺苷含量,依利特Hypersil ODS2(4.6 mm× 250 mm,5μm)色谱柱,流动相为甲醇—水(10∶90),流速0.8mL/min,检测波长260 nm,柱温40℃；RP-HPLC法分析样品中L-焦谷氨酸含量,COSMOSIL 5C18-PAQ(4.6 mm× 250 mm,5μm)色谱柱,流动相为甲醇—5 mmol/L醋酸钠水溶液(8∶92),流速0.5 mL/min,检测波长210 nm,柱温40℃.测得鲜人参及其加工品中腺苷含量为0.332 ～ 0.457 mg/g,L-焦谷氨酸含量为0.315 ～1.056 mg/g.结果表明:腺苷和L焦谷氨酸含量在鲜人参及其加工品中呈规律性变化,鲜人参中腺苷和L-焦谷氨酸含量最高,加工品次之.因此在抗糖尿病活性方面,鲜人参较其加工品应更具研究价值.%This study is to establish the methods for assaying adenosine and L-pyroglutamic acid in ginseng. Six-year-old fresh ginseng, sun-dried ginseng and red ginseng were used as materials. RP- HPLC was used to analyze adenosine in samples. Separation was carried out using a chromatographic column (Hypersil ODS2, 4.6 mm×250 mm, 5 μm). Column temperature and detection wavelength were set at 40℃ and 260 nm, respectively. Mobile phase containing methanol-water (10:90) was used and the flow rate was 0.8 mL/min. Chromatographic column (COSMOSIL 5C18-PAQ, 4.6 mm×250 mm, 5 μm) was applied to analyze L-pyroglutamic acid in ginseng. Mobile phase contained methanol-sodium acetate water solution of 5 mmol/L (8:92), and the flow rate was 0.5 mL/min. Column temperature and detection wavelength for analyzing L-pyroglutamic acid were set at 40℃ and 210 nm, respectively. Adenosine in fresh ginseng and its processed products ranged from 0.332 mg/g to 0.457 mg/g, the amount of L-pyroglutamic acid ranged from 0.315 mg/g to 1.056 mg/g. The results showed that: the amount of adenosine and L-pyroglutamic acid showed regular changes in fresh ginseng and its processed products. Substances of adenosine and L-pyroglutamic in fresh ginseng showed the maximum in different ginseng products, processed products was secondary. Therefore, fresh ginseng possesses more research value than its processed products for anti-diabetic activity.
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