人参皂苷20（S）-Rg3，20（R）-Rg3，Rg5，Rk1对照品的制备∗

摘要

A simple, fast and efficient method for the preparation of ginsenosides 20( S)⁃Rg3, 20 ( R)⁃Rg3 , Rg5 and Rk1 was constructed. Using macroporous absorption resin and silica gel column chromatography, the samples were pretreated. Through RP⁃HPLC, target components 20(S)⁃Rg3, 20( R)⁃Rg3 , Rg5 and Rk1 were rapidly separated from the methanol extracts of red ginseng with the purity over 98%. The separation was performed on a Polaris C18 (250 mm × 4�6 mm, 10 μm) at the room temperature by mobile phase of CH3CN ∶ H2O (46 ∶ 54) at a flow rate of 4�0 mL/min and the detection wavelength was 203 nm. Under these conditions, the yield rates of four ginsenosides were 0�012%, 0�013%, 0�012% and 0�013%, respectively. This method is effective and reliable for the preparation of 20( S)⁃Rg3 , 20( R)⁃Rg3 ,Rg5 and Rk1 from red ginseng.%为建立一种简单快速、高效制备人参皂苷20（ S）⁃Rg3、20（ R）⁃Rg3、Rg5、Rk1的方法。利用大孔吸附树脂和硅胶柱层析对样品进行预处理，通过反相高效制备液相色谱，从红参的甲醇提取物中快速分离得到目标产物人参皂苷20（S）⁃Rg3、20（R）⁃Rg3、Rg5、Rk1，经检测，4种化合物的纯度均＞98％。色谱柱为Polaris C18（250 mm ×4�6 mm，10μm），以V（乙腈）∶ V（水）＝46∶54为流动相，恒梯度洗脱，流速4�0 mL／min，柱温室温，检测波长为203 nm，在此色谱条件下，它们得率分别为0�012％、0�013％、0�012％、0�013％。该方法操作简便，可重复进样，适用于制备高纯度稀有人参皂苷20（ S）⁃Rg3、20（ R）⁃Rg3、Rg5、Rk1。