为研究FTY720诱导K562细胞凋亡与线粒体途径的关系。体外培养人白血病K562细胞，用不同浓度FTY720处理细胞24 h，台盼蓝染色法检测K562细胞存活率；流式细胞术分析细胞凋亡和线粒体功能。免疫印迹技术检测线粒体相关蛋白Bcl⁃2和Bax的表达。收集FTY720处理48 h细胞，流式细胞技术检测细胞周期。结果表明：随FTY720浓度的增加， K562细胞的存活率下降，细胞凋亡增加，线粒体膜电位下降，促凋亡蛋白Bax表达明显增加。细胞周期分析发现，SubG1期细胞增加，而S期和G2M期细胞减少。表明线粒体途径参与FTY720诱导K562细胞凋亡。%The studyaims to clarify the relationship between mitochondrial pathway and apoptosis of K562 cells induced by FTY720. Human leukemia ( K562 cells) were cultured in vitro and treated with different concentrations of FTY720 for 24 h, and then survival rate of K562 cells was detected by trypan blue staining method; apoptosis and mitochondrial function were tested by flow cytometry and expression of Bcl⁃2 and Bax related to mitochondria was detected by Western blotting. K562 cells induced by FTY720 for 48 h were collected and cell cycle was measured by flow cytometry. Through the study, we found that with the increase of concentration of FTY720, survival rate of K562 cells was decreased, apoptosis was increased, mitochondrial membrane potential was de⁃creased , and expression of apoptosis promoting protein Bax was significantly increased . Cell cycle a⁃nalysis showed that percentage of SubG1 phase cells was increased while S phase and phase G2M cells were decreased. The study showed that mitochondrial pathwaywas were involved in apoptosis of K562 cells induced by FTY720.
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