来曲唑对人子宫肌瘤组织中MMP-2和TIMP-2表达的影响

摘要

Objective:To investigate the effect of letrozole on matrix metalloproteinases-2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in the patients with uterine fibroids. Methods: Forty patients with uterine fibroids were divided into two groups. Treatment group patients were received myomectomy after treatment with letrozole tablets (2.5 mg/day) for three course of treatment. Control group were only received myomectomy. The volume of uterus and uterine fibroids were measured through B-mode ultrasonography. The expression of MMP-2 and TIMP-2 in uterine smooth muscle and uterine fibroids were detected by Real-time PCR and Western Blot. Results:Compared with control group and in the treatment group, the clinical symptom was significantly improved, the level of estradiol was significantly decreased, the volume of uterus and uterine fibroids were markedly diminished,the expression of MMP-2 in uterine smooth muscle and u-terine fibroids was significantly decreased, the expression of TIMP-2 in uterine smooth muscle and uterine fibroids was markedly increased in the letrozole group. Conclusions:Letrozole diminishs the size of uterine fibroids in human,the mechanism which of may be related to the decrease of estradiol and MMP-2 expression and the increase of TIMP-2 expression induced by letrozole.%目的:探讨来曲唑对人子宫肌瘤组织中基质金属蛋白酶2(MMP-2)和金属蛋白酶组织抑制因子2(TIMP-2)表达的影响.方法:选择40例子宫肌瘤患者,分为观察组和对照组.观察组患者给予来曲唑片3个疗程后行子宫肌瘤切除术,对照组直接施行子宫肌瘤切除术.超声测量子宫和子宫肌瘤的体积,实时定量聚合酶链反应和免疫印迹法检测子宫肌层和肌瘤组织中MMP-2和TIMP-2的表达.结果:与来曲唑治疗前或与对照组相比较,来曲唑治疗后临床症状显著改善,血清雌二醇水平显著降低,子宫和肌瘤体积均明显缩小,肌层和肌瘤组织中MMP-2的表达降低,肌层和肌瘤组织中TIMP-2的表达增加.结论:来曲唑可缩小子宫肌瘤患者的肌瘤体积,其作用机制可能为来曲唑降低血清雌二醇水平,降低肌层和肌瘤组织中MMP-2的表达,增加肌层和肌瘤组织中TIMP-2的表达.