Objective: Using human trophoblast HTR-8/SVneo (HTR) cell lines for the study of the carrier to study Schisandrin B (Sch B)′s action of benzo [a]pyrene (BaP) damage effect on HTR cell lines aggressivity. Methods:Experiment is divided into the control group, the BaP infected group, different dose Sch B (0.5, 1, 2 μmol/L), intervention infected groupcell proliferation by MTS experimental detection, clear BaP canister and Sch B intervention dose, by Transwell invasion experiment after the detection of BaP infected cells and Sch B cell invasion force change after intervention. Results: ①The MTS test cell proliferation,compared with control group, 20 μmol/L BaP concentration cell proliferation significantly decreased after effect (P<0.01), after Sch B (0.5, 1, 2μmol/L) intervention the cell proliferation is obviously better than BaP infected group (P<0.05).②Transwell cell invasion experiment, compared with control group, the BaP cells infected group was much less aggressive (P<0.05). Sch B intervention group cell invasion force also declined (P<0.05). Compared with the infected group, Sch B cells of aggressive intervention group was obviously ascending (P<0.05). Conclusions:①A certain concentrations of BaP damaged HTR cell proliferation and invasion force. ②Sch B can prevent the damage of BaP of HTR cell proliferation, and at the same time improve cell aggressivity.%目的：以人绒毛膜外滋养层细胞（human trophoblast HTR-8/SVneo，简称HTR）细胞株为研究载体，研究五味子乙素（Schisandrin B，Sch B）对苯并芘（benzo[a]pyrene，BaP）致HTR细胞侵袭力损伤的影响。方法：实验分为对照组、BaP染毒组、不同浓度Sch B（0.5，1，2μmol/L）干预染毒组，通过新型四唑化合物（MTS）法检测细胞增殖情况，明确BaP染毒及Sch B干预剂量；通过Transwell侵袭实验检测BaP染毒后以及Sch B干预后细胞侵袭力的改变。结果：①MTS检测细胞增殖情况：与对照组相比，20μmol/L浓度BaP作用后细胞增殖明显下降（P＜0.01），不同浓度Sch B （0.5，1，2μmol/L）干预后细胞增殖情况优于BaP染毒组（P＜0.05）。②Transwell细胞侵袭实验：与对照组相比，BaP染毒组细胞侵袭力明显下降（P＜0.01），不同浓度Sch B干预组细胞侵袭力也呈下降趋势（P＜0.05）；与BaP染毒组相比，Sch B干预组细胞侵袭力明显提升（P＜0.05）。结论：一定浓度的BaP会对HTR的细胞增殖及侵袭力造成损伤，Sch B可以预防BaP对HTR细胞增殖造成的损伤，同时提高细胞侵袭力。
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